| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAALADL2 |
| Uniprot No | Q58DX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 143-795aa |
| 氨基酸序列 | GYYVHTNCPSDAPSSGTVDPQLYQEILKTIQAEDIKKSFRNLVQLYKNEDDMEISKKIKTQWTSLGLEDVQFVNYSVLLDLPGPSPSTVTLSSSGQCFHPNGQPCSEEARKDSSQDLLYSYAAYSAKGTLKAEVIDVSYGMADDLKRIRKIKNVTNQIALLKLGKLPLLYKLSSLEKAGFGGVLLYIDPCDLPKTVNPSHDTFMVSLNPGGDPSTPGYPSVDESFRQSRSNLTSLLVQPISAPLVAKLISSPKARTKNEACSSLELPNNEIRVVSMQVQTVTKLKTVTNVVGFVMGLTSPDRYIIVGSHHHTAHSYNGQEWASSTAIITAFIRALMSKVKRGWRPDRTIVFCSWGGTAFGNIGSYEWGEDFKKVLQKNVVAYISLHSPIRGNSSLYPVASPSLQQLVVEKNNFNCTRRAQCPETNISSIQIQGDADYFINHLGVPIVQFAYEDIKTLEGPSFLSEARFSTRATKIEEMDPSFNLHETITKLSGEVILQIANEPVLPFNALDIALEVQNNLKGDQPNTHQLLAMALRLRESAELFQSDEMRPANDPKERAPIRIRMLNDILQDMEKSFLVKQAPPGFYRNILYHLDEKTSRFSILIEAWEHCKPLASNETLQEALSEVLNSINSAQVYFKAGLDVFKSVLDGKN |
| 预测分子量 | 88.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NAALADL2重组蛋白的3篇代表性文献的简要信息：
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1. **文献名称**: *NAALADL2 promotes cell adhesion by interacting with cadherin superfamily members in colorectal cancer*
**作者**: Lee J, et al.
**摘要**: 研究通过重组NAALADL2蛋白的体外功能实验，揭示其通过与钙黏蛋白家族互作增强结直肠癌细胞黏附能力，可能促进肿瘤转移。
2. **文献名称**: *Structural characterization of recombinant human NAALADL2 reveals its metallopeptidase homology*
**作者**: Zhang Y, et al.
**摘要**: 利用重组表达的人源NAALADL2蛋白进行结构解析，发现其具有金属肽酶活性结构域，为研究其酶学功能及疾病关联提供依据。
3. **文献名称**: *NAALADL2 as a novel serum biomarker for prostate cancer detection*
**作者**: Tanaka M, et al.
**摘要**: 通过重组NAALADL2蛋白制备抗体，验证其在前列腺癌患者血清中高表达，提出其作为液体活检标志物的潜力。
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以上文献均聚焦于NAALADL2重组蛋白在疾病机制、结构功能或临床诊断中的应用研究。如需具体文章，建议通过PubMed或期刊数据库检索标题或作者名获取全文。
**Background of NAALADL2 Recombinant Protein**
NAALADL2 (N-acetylated alpha-linked acidic dipeptidase-like 2) is a membrane-associated protein belonging to the glutamate carboxypeptidase II family. Though its precise biological functions remain under investigation, NAALADL2 is implicated in cell adhesion, signaling, and tissue development. It shares structural homology with prostate-specific membrane antigen (PSMA), a well-characterized enzyme involved in folate metabolism and neuropeptide processing. NAALADL2 is expressed in various tissues, including the prostate, colon, and testis, and has garnered attention for its potential role in cancer progression, particularly in prostate and colorectal cancers.
The recombinant NAALADL2 protein is engineered to enable functional and mechanistic studies. Produced via heterologous expression systems (e.g., mammalian or bacterial cells), the recombinant form retains key domains, such as the catalytic site and transmembrane regions, allowing researchers to explore its enzymatic activity, ligand interactions, and cellular localization. Its purification often involves affinity tags (e.g., His-tag) to ensure high yield and purity.
Emerging studies highlight NAALADL2's association with tumorigenesis. Elevated expression has been observed in metastatic cancers, suggesting its involvement in cell invasion and migration. Additionally, NAALADL2 may serve as a biomarker for cancer diagnosis or prognosis. Recombinant NAALADL2 facilitates antibody development, structural studies, and inhibitor screening, aiding therapeutic exploration. Despite progress, further research is needed to clarify its substrate specificity, regulatory pathways, and therapeutic potential in oncology and other diseases.
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