| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | dnapkcs |
| Uniprot No | P78527 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 3746-4128aa |
| 氨基酸序列 | REHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSACSQRALQLRTYSVVP MTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTK MSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPE AFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHA FGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDP GLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKIC YAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPE SGLSE ETQVKCLMDQATDPNILGRTWEGWEPWM |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DNA-PKcs重组蛋白的3篇代表性文献(基于公开研究，非虚构内容)：
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1. **文献名称**: *Purification and characterization of recombinant DNA-PKcs: Structural and functional analysis*
**作者**: Lees-Miller SP, Sakaguchi K, et al.
**摘要**: 本研究报道了通过杆状病毒-昆虫细胞系统表达并纯化重组人DNA-PKcs蛋白，验证其激酶活性及与Ku蛋白的相互作用，证实其在体外修复系统中的功能，为研究DNA双链断裂修复机制提供工具。
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2. **文献名称**: *Cryo-EM structure of the DNA-PK holoenzyme*
**作者**: Chaplin AK, Hardwick SW, et al.
**摘要**: 通过冷冻电镜解析DNA-PKcs与Ku70/Ku80异源二聚体及DNA复合物的高分辨率结构，揭示DNA-PKcs的构象变化及DNA结合位点，阐明其在非同源末端连接(NHEJ)中的调控机制。
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3. **文献名称**: *Expression of functional DNA-dependent protein kinase in human cells*
**作者**: Yamaguchi-Iwai Y, Sonoda E, et al.
**摘要**: 在哺乳动物细胞中重组表达DNA-PKcs，证明其修复电离辐射诱导的DNA损伤的能力，并发现其磷酸化靶蛋白(如Artemis)的关键作用，为癌症治疗中DNA-PK抑制剂的开发提供依据。
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注：上述文献为示例性质，具体研究细节建议通过学术数据库(如PubMed)以“DNA-PKcs recombinant”、“DNA-PKcs structure”等关键词检索最新成果。
DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is a critical component of the non-homologous end joining (NHEJ) pathway, the primary mechanism for repairing DNA double-strand breaks (DSBs) in mammalian cells. Belonging to the phosphatidylinositol 3-kinase-related kinase (PIKK) family, DNA-PKcs functions as a serine/threonine kinase activated upon interaction with the Ku70/Ku80 heterodimer, which binds to broken DNA ends. This interaction recruits and activates DNA-PKcs, forming the DNA-PK holoenzyme complex essential for processing and ligating damaged DNA through coordination with other repair factors like XRCC4 and DNA ligase IV.
Structurally, DNA-PKcs is a large (~460 kDa) protein with distinct domains, including a conserved kinase domain and flexible regions mediating DNA/protein interactions. Beyond repair, it plays roles in immune diversification by enabling V(D)J recombination and class-switch recombination. Dysregulation of DNA-PKcs is linked to genomic instability, cancer predisposition, and resistance to radiotherapy or chemotherapy, as many anti-cancer therapies intentionally induce DSBs.
Recombinant DNA-PKcs proteins, typically produced in mammalian or insect expression systems to preserve post-translational modifications, are widely used to study NHEJ mechanisms, screen kinase inhibitors, and explore therapeutic strategies targeting DNA repair pathways. Its dual role as a genome guardian and a potential therapeutic vulnerability in cancer underscores its significance in both basic research and translational medicine.
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