| 纯度 | >90%SDS-PAGE. |
| 种属 | Serratia |
| 靶点 | hasA |
| Uniprot No | Q54450 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-188aa |
| 氨基酸序列 | MAFSVNYDSSFGGYSIHDYLGQWASTFGDVNHTNGNVTDANSGGFYGGSLSGSQYAISSTANQVTAFVAGGNLTYTLFNEPAHTLYGQLDSLSFGDGLSGGDTSPYSIQVPDVSFGGLNLSSLQAQGHDGVVHQVVYGLMSGDTGALETALNGILDDYGLSVNSTFDQVAAATAVGVQHADSPELLAA |
| 预测分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与hasA重组蛋白相关的参考文献示例(内容为模拟生成，仅供参考)：
1. **文献名称**: "High-yield expression and purification of recombinant HasA protein from *Serratia marcescens* in *Escherichia coli*"
**作者**: Smith J et al.
**摘要**: 研究报道了通过构建pET载体在大肠杆菌中高效表达HasA重组蛋白，优化诱导条件后获得可溶性蛋白，并通过亲和层析纯化，为后续功能研究提供基础。
2. **文献名称**: "Structural insights into heme acquisition by HasA: A recombinant protein crystallography study"
**作者**: Tanaka K et al.
**摘要**: 利用重组HasA蛋白进行X射线晶体学分析，揭示了其与血红素结合的关键结构域及作用机制，阐明了细菌通过HasA摄取铁离子的分子途径。
3. **文献名称**: "HasA as a potential vaccine candidate: Immunogenicity evaluation of recombinant protein in murine models"
**作者**: García-Ramírez M et al.
**摘要**: 通过表达纯化HasA重组蛋白，证明其在小鼠模型中可诱导特异性抗体反应，提示其在抗细菌感染疫苗开发中的潜在应用价值。
4. **文献名称**: "Secretion mechanism of recombinant HasA in *Pseudomonas aeruginosa*: Implications for bacterial iron uptake systems"
**作者**: Li H et al.
**摘要**: 通过构建重组HasA分泌表达系统，揭示了该蛋白在假单胞菌中的外排途径依赖T1SS(Type I Secretion System)，为病原菌铁代谢研究提供新视角。
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**备注**：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索关键词(如"recombinant HasA protein", "heme acquisition", "bacterial iron transport")。
**Background of HasA Recombinant Protein**
The *hasA* gene encodes a heme-binding protein originally identified in *Serratia marcescens*, a Gram-negative bacterium. This protein, HasA (Heme acquisition system A), plays a critical role in bacterial iron uptake by scavenging heme from the environment or host proteins, which is then transported into the cell via specific receptors. Heme serves as a vital iron source for pathogens, enabling survival under iron-limiting conditions during infection.
Recombinant HasA protein is produced through genetic engineering, typically by cloning the *hasA* gene into expression vectors (e.g., *E. coli*) for large-scale production. Its ability to bind heme with high affinity (Kd ~nM range) has made it a valuable tool in studying heme-uptake mechanisms, host-pathogen interactions, and bacterial virulence. Additionally, HasA’s stability and reversible heme-binding properties have spurred interest in biotechnological applications, such as developing heme-sensitive biosensors or affinity tags for protein purification.
In research, recombinant HasA is often used as a model to investigate protein-ligand interactions, structural dynamics of heme-binding proteins, and potential therapeutic targets to disrupt bacterial iron acquisition. Its crystal structure, resolved to atomic detail, reveals a unique “heme pocket” stabilized by hydrogen bonds and hydrophobic interactions, providing insights into evolutionary adaptations for heme scavenging.
Overall, HasA recombinant protein bridges microbiological studies and applied sciences, offering both mechanistic insights into bacterial survival strategies and practical avenues for innovation in biomedicine and biotechnology.
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