| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RALGPS1 |
| Uniprot No | Q5JS13 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-305aa |
| 氨基酸序列 | MYKRNGLMASVLVTSATPQGSSSSDSLEGQSCDYASKSYDAVVFDVLKVTPEEFASQITLMDIPVFKAIQPEELASCGWSKKEKHSLAPNVVAFTRRFNQVSFWVVREILTAQTLKIRAEILSHFVKIAKKLLELNNLHSLMSVVSALQSAPIFRLTKTWALLNRKDKTTFEKLDYLMSKEDNYKRTREYIRSLKMVPSIPYLGIYLLDLIYIDSAYPASGSIMENEQRSNQMNNILRIIADLQVSCSYDHLTTLPHVQKYLKSVRYIEELQKFVEDDNYKLSLRIEPGSSSPRLVSSKEDLAAM |
| 预测分子量 | 50.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RALGPS1重组蛋白的参考文献概览：
1. **标题**：Recombinant expression and functional characterization of RALGPS1: a role in cell adhesion and migration
**作者**：Smith J, et al. (2020)
**摘要**：该研究成功在大肠杆菌中表达并纯化RALGPS1重组蛋白，证实其通过激活RalA GTP酶调控细胞粘附和迁移，为癌症转移机制提供新见解。
2. **标题**：Structural insights into RALGPS1-mediated GTPase signaling
**作者**：Chen L, et al. (2018)
**摘要**：通过X射线晶体学解析RALGPS1重组蛋白结构，揭示其PH结构域与RalB特异性结合的分子机制，为靶向Ral信号通路的药物设计奠定基础。
3. **标题**：RALGPS1 interacts with 14-3-3 proteins to regulate MAPK signaling in response to EGF stimulation
**作者**：Kim D, et al. (2015)
**摘要**：利用重组RALGPS1进行体外结合实验，发现其通过14-3-3蛋白依赖的方式调控EGF诱导的MAPK通路活性，提示其在表皮生长信号中的调节作用。
4. **标题**：Cloning and functional analysis of RALGPS1 in vesicle trafficking
**作者**：Wang H, et al. (2012)
**摘要**：首次报道RALGPS1重组蛋白的哺乳动物细胞表达系统，证明其通过RalA激活参与内吞运输过程，拓展了对细胞膜转运机制的理解。
注：上述文献为模拟示例，实际引用时需核实具体来源及准确性。建议通过PubMed或Web of Science以“RALGPS1 recombinant”“RALGPS1 purification”等关键词检索最新研究。
RALGPS1 (Ral Guanine Nucleotide Exchange Factor with PH Domain and SH3 Binding Motif 1) is a multifunctional protein that plays a critical role in cellular signaling pathways, particularly those involving small GTPases. As a member of the RalGEF family, it acts as a guanine nucleotide exchange factor (GEF) specifically for Ral GTPases (RalA and RalB), facilitating the switch from their inactive GDP-bound state to active GTP-bound forms. This activation is essential for regulating diverse cellular processes, including vesicle trafficking, cytoskeletal reorganization, cell proliferation, and apoptosis. RALGPS1 is distinguished by its unique domain architecture, featuring a PH (pleckstrin homology) domain that mediates membrane interactions and a conserved catalytic RalGEF domain.
The protein is implicated in downstream signaling of Ras superfamily GTPases, linking extracellular signals to intracellular responses. Unlike other RalGEFs (e.g., RalGDS), RALGPS1 lacks a Ras-association domain but can be activated via alternative pathways, such as tyrosine kinase receptors or integrin-mediated adhesion. Its activity influences cancer-related pathways, with studies suggesting roles in tumor growth, metastasis, and resistance to therapy. Dysregulation of RALGPS1 has been observed in certain cancers, highlighting its potential as a therapeutic target or biomarker.
Recombinant RALGPS1 protein, produced through heterologous expression systems (e.g., E. coli or mammalian cells), retains its biochemical activity and is widely used in vitro to study Ral GTPase activation mechanisms, screen inhibitors, or explore signaling crosstalk. Purified variants may include tags (e.g., GST, His-tag) for affinity-based assays. Research on recombinant RALGPS1 continues to unravel its context-dependent roles in normal physiology and disease, particularly in cancer and developmental disorders.
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