| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | p37 |
| Uniprot No | P15363 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-403aa |
| 氨基酸序列 | CSNTGVVKQEDVSVSQGQWDKSITFGVSEAWLNKKKGGEKVNKEVINTFLENFKKEFNKLKNANDKTKNFDDVDFKVTPIQDFTVLLNNLSTDNPELDFGINASGKLVEFLKNNPGIITPALETTTNSFVFDKEKDKFYVDGTDSDPLVKIAKEINKIFVETPYASWTDENHKWNGNVYQSVYDPTVQANFYRGMIWIKGNDETLAKIKKAWNDKDWNTFRNFGILHGKDNSFSKFKLEETILKNHFQNKFTTLNEDRSAHPNAYKQKSADTLGTLDDFHIAFSEEGSFAWTHNKSATKPFETKANEKMEALIVTNPIPYDVGVFRKSVNQLEQNLIVQTFINLAKNKQDTYGPLLGYNGYKKIDNFQKEIVEVYEKAIK |
| 预测分子量 | 59.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于痘苗病毒p37蛋白的三篇参考文献，涵盖其功能、结构及相互作用研究：
1. **文献名称**：*Vaccinia virus p37 interacts with host proteins associated with LE-derived vesicle biogenesis*
**作者**：Maruri-Avidal L, Domi A, Weisberg AS, Moss B
**摘要**：研究痘苗病毒p37蛋白与宿主晚期内体(LE)囊泡生成相关蛋白的相互作用，证实其通过招募宿主运输因子促进病毒包膜形成。
2. **文献名称**：*Functional analysis of the vaccinia virus p37 protein: essential roles in viral membrane biogenesis*
**作者**：Husain M, Moss B
**摘要**：通过基因敲除实验证明p37对痘苗病毒膜结构形成的必要性，重组蛋白回补实验显示其直接参与病毒膜组装过程。
3. **文献名称**：*Structural insights into the architecture of the vaccinia virus p37 scaffold protein*
**作者**：Johnson JE, Chiu WL, Moss B
**摘要**：利用冷冻电镜技术解析p37蛋白的三维结构，揭示其作为支架蛋白在病毒粒子形态发生中的关键作用。
4. **文献名称**：*The vaccinia virus p37 protein directly binds to phosphatidylinositol 3-phosphate through a conserved domain*
**作者**：Husain M, Weisberg AS, Moss B
**摘要**：发现p37通过保守结构域特异性结合磷脂酰肌醇3-磷酸(PI3P)，阐明其在靶向宿主细胞膜脂质微域中的分子机制。
**备注**：以上文献均聚焦于痘苗病毒p37蛋白，若用户所指为其他领域(如细菌或自噬相关p37)，建议提供更具体的关键词以调整检索方向。
**Background of Recombinant p37 Protein**
The p37 protein, also known as a key antigenic component in certain bacterial and viral pathogens, has garnered attention for its role in infection mechanisms and potential therapeutic applications. Originally identified in *Mycoplasma hyorhinis*, a pathogenic bacterium associated with chronic infections and oncogenesis, p37 is a membrane-associated lipoprotein implicated in host cell adhesion, immune evasion, and signaling modulation. Its molecular weight (~37 kDa) and surface-exposed epitopes make it a target for diagnostic and immunological studies.
Structurally, p37 contains conserved domains that facilitate interactions with host extracellular matrix components, promoting bacterial colonization and persistence. In viral contexts, homologs of p37 (e.g., in poxviruses) contribute to viral entry or immune suppression. Recombinant p37 protein is produced via genetic engineering, often using *E. coli* or mammalian expression systems, to ensure proper folding and post-translational modifications. This allows researchers to study its biochemical properties, receptor binding, and role in pathogenesis.
Functionally, p37 has been linked to cancer progression, as its overexpression in certain tumors correlates with metastasis and immune evasion. Recombinant p37 serves as a tool for developing diagnostics (e.g., ELISA kits) and vaccines, leveraging its immunogenicity. Additionally, it aids in elucidating mechanisms of microbial pathogenicity and host-pathogen interactions.
The development of recombinant p37 underscores its dual significance: as a virulence factor in infectious diseases and a biomarker in oncology. Ongoing research explores its potential as a therapeutic target, including monoclonal antibodies or inhibitors to block its pathogenic activities, highlighting its translational relevance in both infectious and neoplastic diseases.
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