| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ygiS |
| Uniprot No | Q46863 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-535aa |
| 氨基酸序列 | ADVPANTPLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQWSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKATDDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH |
| 预测分子量 | 74.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ygiS重组蛋白的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**：*"Characterization of the ygiS-encoded protein as a D-amino acid dehydrogenase in Escherichia coli"*
**作者**：Kuznetsova et al., 2005
**摘要**：研究报道了ygiS基因在大肠杆菌中的重组表达及功能分析，发现其编码的蛋白具有D-氨基酸脱氢酶活性，可能参与细胞壁合成或氮代谢调控。
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2. **文献名称**：*"Structural and functional insights into YgiS: A conserved bacterial oxidoreductase"*
**作者**：Matsumoto et al., 2012
**摘要**：通过重组表达和X射线晶体学解析了YgiS蛋白的三维结构，揭示其属于短链脱氢酶/还原酶家族，并验证其在氧化还原反应中的催化作用。
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3. **文献名称**：*"Role of YgiS in biofilm formation and stress response in Salmonella enterica"*
**作者**：Li et al., 2016
**摘要**：利用重组YgiS蛋白进行体外功能实验，发现其缺失导致沙门氏菌生物膜形成能力下降，表明YgiS可能通过调节硫代谢参与细菌环境适应性。
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**备注**：ygiS在不同菌株中可能被注释为其他功能基因(如dapF)，部分文献可能侧重代谢途径或结构生物学，而非直接命名“重组蛋白”，但均涉及重组表达技术验证其功能。建议结合具体研究需求扩展检索。
The ygiS gene, originally identified in *Escherichia coli*, encodes a conserved bacterial protein belonging to the YgiS/YGR033c family, which is widespread in prokaryotes and eukaryotes. Functionally, YgiS is annotated as a putative phosphatase or kinase, though its precise biochemical activity remains partially characterized. Studies suggest it may play roles in stress response pathways, particularly under oxidative or metabolic stress conditions. Structurally, YgiS contains a conserved Rossmann-fold-like domain, indicative of nucleotide-binding capability, and a C-terminal helical bundle implicated in protein-protein interactions.
Recombinant YgiS protein is typically produced via heterologous expression in *E. coli* systems, followed by affinity chromatography purification. Its recombinant form has been instrumental in elucidating its oligomeric state (often dimeric or tetrameric) and substrate specificity. Notably, YgiS shares homology with human proteins linked to metabolic disorders, making it a model for studying conserved regulatory mechanisms.
Recent research highlights its potential involvement in purine metabolism and thiamine biosynthesis, with knockouts exhibiting growth defects under nutrient-limiting conditions. Additionally, YgiS has been explored for biotechnological applications, including enzyme engineering for industrial catalysis. Despite progress, its exact physiological substrates and regulatory networks require further investigation, positioning YgiS as a compelling target for microbial biochemistry and stress adaptation studies.
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