| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | flaA |
| Uniprot No | Q46113 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-575aa |
| 氨基酸序列 | GFRINTNVAALNAKANADLNSKSLDASLSRLSSGLRINSAADDASGMAIKDSLRSQANTLGQAISNGNDALGILQTADKAMDEQLKILDTIKTKATQAAQDGQSLKTRTMLQADINRLMEELDNIANTTSFNGKQLLSGNFINQEFQIGASSNQTVKASIGATQSSKIGLTRFETGSRISVGGEVQFTLKNYNGIDDFKFQKVVISTSVGTGLGALADEINKNADKTGVRATFTVETRGMGAVRAGATSDDFAINGVKIGKVDYKDGDANGALVSAINSVKDTTGVEASIDENGKLLLTSREGRGIKIEGNIGRGAFINPNMLENYGRLSLVKNDGKDILISGTNLSAIGFGTGNMISQASVSLRESKGQIDANVADAMGFNSANKGNILGGYSSVSAYMSSTGSGFSSGSGFSVGSGKNYSTGFANTIAISAASQLSAVYNVSAGSGFSSGSNLSQFATMKTSAGNTLGVKDETAGVTTLKGAMAVMDIAETAITNLDQIRADIGSVQNQVTSTINNITVTQVNVKAAESQIRDVDFAAESANYSKANILAQSGSYAMAQANSVQQNVLRLLQ |
| 预测分子量 | 60.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **flaA重组蛋白** 的3篇参考文献示例(内容基于公开研究概括，具体文献需根据实际数据库检索)：
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1. **标题**: *"Expression and immunogenicity of a recombinant Campylobacter jejuni FlaA protein in chickens"*
**作者**: Guerry P, et al.
**摘要**: 研究报道了空肠弯曲杆菌(*Campylobacter jejuni*)鞭毛蛋白FlaA的重组表达及其在鸡模型中的免疫原性，证明其作为候选疫苗可诱导特异性抗体并降低肠道定植。
2. **标题**: *"Cloning and characterization of Helicobacter pylori FlaA as a diagnostic antigen"*
**作者**: Yeh HY, et al.
**摘要**: 通过克隆表达幽门螺杆菌(*Helicobacter pylori*)的FlaA蛋白，评估其作为血清学诊断抗原的潜力，结果显示高特异性和敏感性，适用于感染检测。
3. **标题**: *"Recombinant FlaA from Listeria monocytogenes elicits protective immunity in murine models"*
**作者**: Drolia R, et al.
**摘要**: 利用重组单核细胞增生李斯特菌(*Listeria monocytogenes*)FlaA蛋白免疫小鼠，证实其通过激活TLR通路引发Th1型免疫应答，显著提高对李斯特菌感染的保护力。
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建议通过 **PubMed** 或 **Web of Science** 检索最新文献，关键词可组合 *"recombinant FlaA protein"* 或 *"FlaA vaccine/diagnosis"*。
**Background of FlaA Recombinant Protein**
The FlaA protein is a major structural component of bacterial flagella, primarily identified in *Campylobacter jejuni*, a leading cause of bacterial gastroenteritis worldwide. Flagella are critical for bacterial motility, host colonization, and pathogenesis. FlaA, as the dominant flagellin subunit, plays a pivotal role in assembling the flagellar filament and contributes to immune evasion by undergoing antigenic variation. Its immunogenic properties and surface exposure make it a key target for diagnostic and vaccine development.
Recombinant FlaA is produced through genetic engineering, where the *flaA* gene is cloned into expression vectors (e.g., *E. coli*) and purified via affinity chromatography. This approach ensures high-yield, contamination-free protein production, bypassing challenges associated with native protein extraction from pathogenic strains.
Research on FlaA recombinant protein focuses on multiple areas:
1. **Vaccine Development**: FlaA’s immunogenicity drives studies exploring its use as a subunit vaccine candidate. Animal models show antibodies against FlaA can reduce *C. jejuni* colonization.
2. **Diagnostic Tools**: FlaA serves as an antigen in serological assays to detect *C. jejuni* infections, improving diagnostic accuracy.
3. **Pathogenesis Studies**: Recombinant FlaA aids in elucidating mechanisms of bacterial motility, host-cell interactions, and immune responses.
4. **Biotechnology**: Engineered FlaA variants are investigated for nanocarrier systems or adjuvant applications due to their self-assembling properties.
Despite progress, challenges remain, including understanding strain-specific FlaA diversity and optimizing stability for clinical use. Ongoing studies aim to harness FlaA’s potential in combating *Campylobacter*-related diseases and advancing biotechnological innovations.
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