| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | flaB |
| Uniprot No | O51941 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-283aa |
| 氨基酸序列 | MIINHNLSAVNAHRSLKFNELAVDKTMKALSSGMRINSAADDASGLAVSEKLRTQINGLRQAERNTEDGMSFIQTAEGFLEQTSNIIQRIRVLAIQTSNGIYSNEDRQLVQVEVSALVDEVDRIASQAEFNKFKLFEGQFARGSRVASMWFHMGPNQNQRERFYIGTMTSKALKLVKADGRPIAISSPGEANDVIGLADAALTKIMKQRADMGAYYNRLEYTAKGLMGAYENMQASESRIRDADMAEEVVSLTTKQILVQSGTAMLAQANMKPNSVLKLLQQI |
| 预测分子量 | 38.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于flaB重组蛋白的3篇文献示例(注：内容为模拟概括，非真实文献)：
1. **《Expression and purification of recombinant Helicobacter pylori FlaB protein in E. coli》**
- 作者：Wang L, et al.
- 摘要：本研究成功在大肠杆菌中表达并纯化了幽门螺杆菌flaB重组蛋白，优化了表达条件，验证了其作为诊断抗原的潜力。
2. **《FlaB from Helicobacter pylori induces potent immune responses via TLR5 activation》**
- 作者：Smith J, et al.
- 摘要：发现幽门螺杆菌flaB重组蛋白通过激活TLR5通路触发强烈的先天免疫反应，提示其作为疫苗佐剂的应用价值。
3. **《Development of a flaB-based serodiagnostic assay for Helicobacter pylori infection》**
- 作者：Zhang Y, et al.
- 摘要：利用重组flaB蛋白建立新型血清学检测方法，证明其较传统抗原具有更高的敏感性和特异性。
如需具体文献，建议通过PubMed或Google Scholar以“flaB recombinant protein”、“Helicobacter pylori flagellin”等关键词检索。
**Background of FlaB Recombinant Protein**
FlaB, a major flagellin protein, is a key structural component of bacterial flagella, particularly in spirochetes like *Borrelia burgdorferi*, the causative agent of Lyme disease. It plays a critical role in bacterial motility, virulence, and host-pathogen interactions. FlaB is highly conserved across *Borrelia* species and exhibits strong immunogenicity, making it a valuable target for diagnostic and research applications.
Recombinant FlaB is produced via genetic engineering, typically by cloning the *flaB* gene into expression systems like *Escherichia coli*. This allows large-scale purification of the protein while retaining its native antigenic and structural properties. Unlike native flagellin, recombinant FlaB avoids contamination risks associated with pathogen cultivation, ensuring safer handling. Its robust expression and stability further enhance its utility in biomedical studies.
In research, FlaB is widely used to study host immune responses, as it activates Toll-like receptor 5 (TLR5), triggering pro-inflammatory signaling. This property also positions it as a potential vaccine adjuvant. Diagnostically, FlaB serves as a biomarker in serological assays (e.g., ELISA, immunoblot) to detect *Borrelia*-specific antibodies, improving Lyme disease testing accuracy. Additionally, its role in bacterial motility mechanisms offers insights into developing anti-infective therapies. Overall, FlaB recombinant protein bridges microbiological research, clinical diagnostics, and therapeutic innovation.
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