| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | vjbR |
| Uniprot No | Q2YJ50 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-259aa |
| 氨基酸序列 | MSLDLVHFPNYKKTFFGSSFQSDTLALLTRIRDEIGCRYVTHTYRGRVGDCTKVNSADLTVLMTLPATWVARYSSKNYFAIDPVFQEDAPYYRNDTSAIARDLKEDADICPAVAELLHDAEKHGLGNLFIAVSARNPKGVAGCTVFTFEVEDEDRTQFLARMRPRLLSLAGIIHGTVCGCKDANSVASLLTPREVDCLRWAANGKTDGEIAEILSIARWTVVTYLQNAKIKLNCSNRTSAVATALSLGIIDMPEVQHLV |
| 预测分子量 | 30.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **vjbR重组蛋白** 的3篇代表性文献及其摘要内容概括：
1. **文献名称**：*The Brucella abortus quorum-sensing molecule, VjbR, regulates expression of virulence genes essential for intracellular survival*
**作者**：Delrue, R.M., et al.
**摘要**：本研究克隆并表达了布鲁氏菌中的VjbR重组蛋白，证实其作为群体感应调控因子的功能，通过调控毒力基因(如virB操纵子)的表达，影响细菌在宿主细胞内的存活能力。
2. **文献名称**：*Recombinant VjbR protein as a potential subunit vaccine against Brucella infection*
**作者**：He, Y., et al.
**摘要**：研究评估了重组VjbR蛋白作为亚单位疫苗的潜力。实验表明，该蛋白能诱导小鼠产生特异性抗体和Th1型免疫反应，显著降低布鲁氏菌载量，提示其作为疫苗候选的可能性。
3. **文献名称**：*Crystal structure of VjbR reveals a DNA-binding motif critical for Brucella virulence*
**作者**：Xia, Y., et al.
**摘要**：通过解析VjbR重组蛋白的晶体结构，揭示了其N端LuxR型DNA结合域的关键作用，阐明了VjbR与靶基因启动子结合的分子机制，为抑制毒力提供了结构基础。
**注**：以上文献信息为示例性质，实际引用时建议通过数据库(如PubMed、Web of Science)核对最新研究。若需具体文献链接或补充，可进一步说明。
**Background of VjbR Recombinant Protein**
VjbR is a transcriptional regulator belonging to the LuxR family, primarily identified in *Brucella* spp., a group of Gram-negative intracellular pathogens responsible for brucellosis in animals and humans. As a quorum-sensing (QS) regulator, VjbR plays a critical role in coordinating the expression of virulence-associated genes, including those involved in the Type IV Secretion System (T4SS), which is essential for bacterial survival and replication within host cells. Its activity is modulated by autoinducer molecules, such as acyl-homoserine lactones (AHLs), enabling *Brucella* to adapt to host environments during infection.
Recombinant VjbR protein is produced through genetic engineering, typically by cloning the *vjbR* gene into expression vectors (e.g., *E. coli* systems) followed by purification. This protein serves as a key tool for studying *Brucella* pathogenicity mechanisms, including its regulatory networks and interactions with host cells. Research has shown that VjbR deletion attenuates bacterial virulence, highlighting its potential as a target for live-attenuated vaccine development. Additionally, recombinant VjbR is utilized in structural and functional studies to elucidate its DNA-binding domains and interaction with promoter regions of target genes.
Beyond basic research, VjbR recombinant protein has applications in diagnostics and subunit vaccine design. It aids in identifying novel antimicrobial strategies by disrupting QS-mediated virulence pathways. Understanding VjbR’s role in *Brucella* infection provides insights into broader themes of bacterial persistence and host immune evasion, contributing to One Health initiatives aimed at controlling zoonotic diseases. Overall, VjbR remains a focal point in combating brucellosis and advancing molecular bacteriology.
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