| 纯度 | >90%SDS-PAGE. |
| 种属 | Bovine |
| 靶点 | PAG2 |
| Uniprot No | Q28057 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-376aa |
| 氨基酸序列 | KKMKTLRETLREKNLLNNFLEEQAYRLSKNDSKITIHPLRNYLDTAYVGNITIGTPPQEFRVVFDTGSANLWVPCITCTSPACYTHKTFNPQNSSSFREVGSPITIFYGSGIIQGFLGSDTVRIGNLVSPEQSFGLSLEEYGFDSLPFDGILGLAFPAMGIEDTIPIFDNLWSHGAFSEPVFAFYLNTNKPEGSVVMFGGVDHRYYKGELNWIPVSQTSHWQISMNNISMNGTVTACSCGCEALLDTGTSMIYGPTKLVTNIHKLMNARLENSEYVVSCDAVKTLPPVIFNINGIDYPLRPQAYIIKIQNSCRSVFQGGTENSSLNTWILGDIFLRQYFSVFDRKNRRIGLAPAV |
| 预测分子量 | 42.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PAG2重组蛋白的3篇代表性文献的简要信息：
1. **文献名称**：*PAG2 regulates T-cell receptor signaling through recruitment of inhibitory phosphatases*
**作者**：Lind et al.
**摘要**：研究揭示了PAG2重组蛋白在T细胞激活中的作用，通过结合磷酸酶(如SHP-1)负向调控TCR信号通路，抑制过度免疫反应。
2. **文献名称**：*Recombinant PAG2 expression in E. coli and its functional characterization*
**作者**：Smith et al.
**摘要**：报道了利用大肠杆菌系统高效表达可溶性PAG2重组蛋白的方法，并验证其与细胞膜蛋白的相互作用，为后续机制研究提供工具。
3. **文献名称**：*PAG2 modulates EGFR endocytosis in cancer cells via ubiquitination pathways*
**作者**：Chen & Wang
**摘要**：通过体外重组PAG2蛋白实验，证明其通过调控EGFR泛素化影响肿瘤细胞的内吞过程，可能成为癌症治疗的潜在靶点。
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注：以上文献为示例，实际文献需通过数据库(如PubMed、Web of Science)检索确认。研究重点可围绕PAG2的免疫功能、重组表达技术或疾病机制展开。
**Background of PAG2 Recombinant Protein**
Pregnancy-associated glycoprotein 2 (PAG2) belongs to the PAG family, a group of placenta-expressed proteins evolutionarily linked to aspartic proteinases. Initially identified in ruminants, PAGs are implicated in maternal-fetal communication, immune modulation, and placental development. PAG2. specifically, is secreted by trophoblast cells during early gestation and is considered a marker of placental health. Its structural homology to pepsin-like enzymes suggests potential enzymatic or ligand-binding roles, though its exact biological function remains under investigation.
Recombinant PAG2 is produced using genetic engineering techniques, often expressed in bacterial or mammalian systems to ensure proper post-translational modifications. This allows large-scale production of the protein for research and diagnostic applications. Studies highlight its utility in reproductive biology, particularly in understanding pregnancy disorders like preeclampsia or miscarriage, where PAG2 levels may correlate with placental dysfunction. Additionally, PAG2 has been explored in oncology due to its aberrant expression in certain cancers, suggesting roles in tumor immune evasion or metastasis.
The development of recombinant PAG2 has also advanced serological assays for monitoring pregnancy in livestock, improving breeding efficiency. In humans, it holds promise as a non-invasive biomarker for early pregnancy complications. Ongoing research aims to unravel its molecular interactions, including binding partners and signaling pathways, to clarify its physiological and pathological significance. Overall, PAG2 recombinant protein serves as a critical tool in both basic science and clinical applications, bridging gaps in reproductive health and disease mechanisms.
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