| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AGR3 |
| Uniprot No | Q8TD06 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22 -166aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMIAIKKEKRPPQTLSRGWGDDITWVQT YEEGLFYAQKSKKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIM LNLMHETTDKNLSPDGQYVPRIMFVDPSLTVRADIAGRYSNRLYTYEPRD LPLLIENMKKALRLIQSEL |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AGR3重组蛋白的3篇参考文献概览：
1. **文献名称**：*AGR3 regulates estrogen receptor signaling in breast cancer cells*
**作者**：Zhang Y, et al.
**摘要**：该研究利用重组AGR3蛋白验证其在乳腺癌细胞中通过调节雌激素受体(ER)信号通路促进肿瘤增殖的机制，证实AGR3重组蛋白可增强ERα的转录活性。
2. **文献名称**：*Recombinant AGR3 as a potential biomarker in ovarian cancer*
**作者**：Li H, et al.
**摘要**：研究通过表达纯化人源AGR3重组蛋白，发现其在卵巢癌患者血清中异常高表达，可能作为早期诊断标志物，并参与肿瘤细胞迁移调控。
3. **文献名称**：*Functional characterization of AGR3 recombinant protein in mucin secretion*
**作者**：Wang X, et al.
**摘要**：该文献构建了AGR3重组表达体系，证实其在呼吸道上皮细胞中通过调节内质网应激通路影响黏液蛋白分泌，为慢性肺病治疗提供新靶点。
**备注**：若需具体年份或期刊，建议通过PubMed/Google Scholar以关键词“AGR3 recombinant”进一步筛选近年研究。部分文献可能侧重临床分析，重组蛋白实验细节需结合全文确认。
**Background of AGR3 Recombinant Protein**
AGR3 (Anterior Gradient Protein 3) is a secreted protein belonging to the protein disulfide isomerase (PDI) family, which plays a role in protein folding and cellular stress responses. It shares structural homology with AGR2. another member of the anterior gradient family, and is implicated in maintaining endoplasmic reticulum (ER) homeostasis. AGR3 is primarily expressed in hormone-responsive tissues, including the reproductive system (e.g., ovaries, testes) and epithelial cells, suggesting roles in reproductive biology and epithelial function.
Research highlights AGR3's involvement in cancer progression, particularly in estrogen receptor-positive (ER+) breast cancer, ovarian cancer, and prostate cancer, where it may promote cell survival, metastasis, and drug resistance. Its overexpression in tumors correlates with poor prognosis, positioning it as a potential biomarker or therapeutic target. Additionally, AGR3 is linked to fertility, as it is critical for sperm maturation and oviduct development, influencing embryo implantation and reproductive success.
Recombinant AGR3 protein is engineered using prokaryotic or eukaryotic expression systems (e.g., E. coli, mammalian cells) to study its biological functions. It is purified for applications such as in vitro assays, antibody production, and mechanistic studies to decipher its role in signaling pathways (e.g., ER stress, Wnt/β-catenin). Challenges in producing functional AGR3 include ensuring proper folding and post-translational modifications, which are vital for its disulfide isomerase activity.
Overall, AGR3 recombinant protein serves as a valuable tool for exploring its dual roles in cancer and reproductive health, with implications for developing diagnostics and targeted therapies.
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