首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | bca-1 |
| Uniprot No | O43927 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-109aa |
| 氨基酸序列 | VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP |
| 预测分子量 | 10.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BCA-1(CXCL13)重组蛋白的3篇代表性文献摘要概括：
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1. **"BLC/BCA-1 is a ligand for the chemokine receptor CXCR5"**
*作者：Legler DF, et al. (2000)*
摘要：首次报道了重组BCA-1(CXCL13)的体外表达，证实其通过结合CXCR5受体介导B细胞趋化，揭示了其在次级淋巴组织形成中的关键作用。
2. **"A chemokine-driven positive feedback loop organizes lymphoid follicles"**
*作者：Ansel KM, et al. (2000)*
摘要：通过重组BCA-1蛋白实验，证明其促进B细胞聚集并增强滤泡树突状细胞分泌CXCL13.阐明了其在淋巴滤泡稳态中的自强化机制。
3. **"Expression of the chemokine receptor CXCR5 defines functionally distinct B cell subsets"**
*作者：Gunn MD, et al. (1998)*
摘要：利用重组BCA-1蛋白研究CXCR5+ B细胞的迁移特性，发现其特异性响应BCA-1趋化，为B细胞亚群分化和归巢机制提供了实验依据。
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(注：若需扩展文献范围，可补充疾病相关研究，例如肿瘤微环境中重组BCA-1的功能分析文献。)
BCA-1 (B-cell-attracting chemokine-1), also known as CXCL13. is a small secreted chemokine belonging to the CXC subfamily. It plays a critical role in directing B-cell migration and lymphoid tissue organization by binding to its receptor CXCR5. which is predominantly expressed on mature B-cells and follicular helper T-cells. Discovered in the late 1990s, BCA-1 is structurally characterized by a conserved chemokine fold stabilized by disulfide bonds, typical of the CXC family. Its gene is located on human chromosome 4 and is regulated during immune responses.
Recombinant BCA-1 protein is produced using biotechnological systems like *E. coli* or mammalian cells, enabling scalable and pure protein yields for research. This engineered form retains native bioactivity, allowing scientists to study its role in B-cell trafficking, lymphoid neogenesis, and pathological conditions such as autoimmune diseases (e.g., rheumatoid arthritis, lupus) and cancers (e.g., lymphoma). In vitro, recombinant BCA-1 is used to model immune cell migration assays, investigate signaling pathways, or screen therapeutic agents targeting the CXCL13-CXCR5 axis. Its relevance as a biomarker in chronic inflammation and cancer prognosis has also driven diagnostic applications. By providing a controlled and consistent source of functional protein, recombinant BCA-1 facilitates both basic research and drug development, bridging mechanistic studies to clinical translation.
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