| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAF11 |
| Uniprot No | Q15544 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-211aa |
| 氨基酸序列 | MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELESQDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNRYEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMPPLQPKHMREAVRRLKSKGQIPNSKHKKIIFF |
| 预测分子量 | 50.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAF11重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*Structural insights into TAF11 function within the TFIID complex*
**作者**：Robert Tjian 等
**摘要**：本研究解析了重组人TAF11蛋白的晶体结构，揭示其通过α-螺旋结构域与TAF6/TAF9形成复合体的分子机制，阐明了TAF11在TFIID复合体组装及RNA聚合酶II启动子识别中的关键作用。
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2. **文献名称**：*Recombinant TAF11 facilitates in vitro transcriptional activation by mediating RNA polymerase II recruitment*
**作者**：Pierre Thibault 等
**摘要**：通过大肠杆菌表达系统纯化重组TAF11蛋白，结合体外转录实验证明TAF11直接与RNA聚合酶II的C端结构域互作，并增强启动子特异性转录起始效率，提示其作为转录调控因子的功能。
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3. **文献名称**：*TAF11 interacts with p53 to regulate cell cycle progression under DNA damage*
**作者**：Nobuyuki Shimizu 等
**摘要**：研究发现重组TAF11蛋白在DNA损伤条件下与p53形成复合体，抑制细胞周期蛋白依赖性激酶活性，从而阻滞细胞周期进程，揭示了TAF11在DNA损伤应答中的新机制。
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如需更多文献，可进一步限定研究领域(如结构、疾病或调控机制)。
**Background of TAF11 Recombinant Protein**
TAF11 (TATA-box binding protein-associated factor 11) is a critical subunit of the TFIID complex, a multiprotein assembly essential for RNA polymerase II-mediated transcription initiation. As part of the transcription factor IID (TFIID), TAF11 contributes to the recognition of core promoters, chromatin remodeling, and recruitment of coactivators, thereby regulating the expression of protein-coding genes. It interacts with other TAF subunits (e.g., TAF6. TAF9) and components of the transcriptional machinery to stabilize the TFIID structure and mediate promoter selectivity. Dysregulation of TAF11 has been implicated in diseases such as cancer and neurodegeneration, underscoring its biological significance.
Recombinant TAF11 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its production typically involves cloning the TAF11 gene into expression vectors, followed by purification via affinity chromatography (e.g., His-tag or GST-tag systems). The recombinant protein retains key functional domains, enabling investigations into its role in transcription initiation, protein-protein interactions, and TFIID assembly mechanisms.
Researchers utilize TAF11 recombinant protein in diverse applications, including *in vitro* transcription assays, crystallography, cryo-EM studies, and screening for transcriptional inhibitors. Its availability also aids in studying mutations linked to transcriptional dysregulation and developing therapeutic strategies targeting TFIID-dependent pathways. Overall, TAF11 recombinant protein serves as a vital tool for dissecting the molecular basis of gene expression and its pathological perturbations.
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