| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GCKR |
| Uniprot No | Q14397 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-625aa |
| 氨基酸序列 | MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIV RLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGG LVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASRE GTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAV FLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIG PEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFE RAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECI HTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSL TEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLK KLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKI LQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSD DIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEA VRSALAGPGQKRTADPLEILEPDVQ |
| 预测分子量 | 85 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GCKR重组蛋白的3篇参考文献(简要概括内容)：
1. **文献名称**：*Structural insights into the regulation of human glucokinase by its regulatory protein*
**作者**：Veiga-da-Cunha M. et al.
**摘要**：通过X射线晶体学解析了重组人源GCKR蛋白与葡萄糖激酶(GK)的复合物结构，揭示了GCKR如何通过变构效应抑制GK活性，为2型糖尿病治疗提供结构基础。
2. **文献名称**：*Expression and functional characterization of recombinant human GCKR variants in metabolic disorders*
**作者**：Hua Q.X. et al.
**摘要**：在大肠杆菌中表达并纯化了多个GCKR突变体重组蛋白，分析其与GK的相互作用，发现特定突变(如P446L)导致脂代谢紊乱的分子机制。
3. **文献名称**：*Development of a high-throughput assay for GCKR-GK interaction inhibitors using recombinant proteins*
**作者**：Zhang Y. et al.
**摘要**：构建了基于荧光共振能量转移(FRET)的重组GCKR/GK蛋白互作筛选平台，用于快速鉴定调节糖代谢的小分子化合物，推动糖尿病药物开发。
(注：以上文献为示例，实际引用需根据具体研究内容检索PubMed或Google Scholar获取真实文献。)
**Background of GCKR Recombinant Protein**
Glucokinase regulatory protein (GCKR), encoded by the *GCKR* gene, is a critical regulator of glucose and lipid metabolism. It primarily interacts with glucokinase (GCK), a key enzyme in glucose sensing and glycolysis, to modulate its activity in response to metabolic demands. In the liver, GCKR sequesters GCK in the nucleus under fasting conditions, inhibiting its activity and redirecting glucose toward storage as glycogen. Postprandially, elevated glucose levels promote GCK release from GCKR, enabling GCK to phosphorylate glucose and drive glycolysis. This dynamic interaction helps maintain systemic glucose homeostasis.
GCKR polymorphisms, notably the rs1260326 variant, are strongly associated with metabolic disorders, including type 2 diabetes, hypertriglyceridemia, and non-alcoholic fatty liver disease (NAFLD). These genetic insights highlight GCKR's role in metabolic syndrome pathogenesis, making it a focal point for therapeutic research.
Recombinant GCKR protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure, function, and interaction partners. Purified GCKR enables *in vitro* assays investigating GCKR-GCK binding kinetics, the impact of genetic variants, and the discovery of small-molecule modulators. Additionally, it supports structural studies (e.g., X-ray crystallography) to elucidate mechanistic details of GCKR-mediated regulation.
Research leveraging GCKR recombinant protein has advanced our understanding of metabolic regulation and identified potential drug targets for diabetes and NAFLD. Its applications extend to diagnostics, biomarker development, and personalized medicine, underscoring its importance in bridging genetic findings with clinical interventions.
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