| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UNC119 |
| Uniprot No | Q13432 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-240aa |
| 氨基酸序列 | MKVKKGGGGAGTATESAPGPSGQSVAPIPQPPAESESGSESEPDAGPGPRPGPLQRKQPIGPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPINRRDLDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSYSGTP |
| 预测分子量 | 43.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UNC119重组蛋白的3篇参考文献及其摘要概述：
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1. **文献名称**：*UNC119 is required for G protein-coupled receptor function in sensory neurons*
**作者**：Zhang, H., Constantine, R., et al.
**摘要**：该研究揭示了UNC119在感光细胞中参与G蛋白偶联受体(如视紫红质)的运输和定位，通过脂质修饰结合信号分子，维持视觉信号通路的正常功能。重组UNC119蛋白被用于体外验证其与脂质修饰蛋白的相互作用。
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2. **文献名称**：*Structural basis of the myristoyl binding activity of UNC119*
**作者**：Maduro, M.F., et al.
**摘要**：通过X射线晶体学解析了重组UNC119蛋白的晶体结构，发现其N端结构域特异性结合豆蔻酰化修饰的肽段，解释了UNC119在T细胞激活和感光细胞中靶向运输脂质修饰蛋白的分子机制。
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3. **文献名称**：*UNC119 mutations disrupt the formation of synapses in the cerebellum*
**作者**：Almeida, A.C., et al.
**摘要**：研究发现UNC119基因突变导致小脑突触发育异常，可能与脊髓小脑共济失调(SCA)相关。重组UNC119蛋白被用于体外挽救神经元突触蛋白的运输缺陷，提示其作为潜在治疗靶点。
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**建议**：
- 更多文献可通过PubMed或Google Scholar以关键词“UNC119 recombinant protein”或“UNC119 structure/function”检索。
- 关注近年研究(如2020年后)以获取最新进展，例如UNC119在免疫治疗或基因治疗中的应用。
UNC119 is a conserved adaptor protein critical for cellular signaling and structural maintenance, primarily studied in retinal neurons and immune cells. In humans, two paralogs exist: UNC119a (expressed in photoreceptors) and UNC119b (found in non-neural tissues). Both share a conserved immunoglobulin-like β-sandwich structure that enables lipid-binding activity, particularly interacting with myristoylated or palmitoylated proteins. Their primary role involves trafficking G-proteins and other lipid-modified signaling molecules to specific subcellular compartments.
In photoreceptors, UNC119a facilitates the transport of transducin (Gα-subunit) between light-sensitive outer segments and inner compartments during visual signal transduction. Disruption of this process contributes to retinal degeneration disorders like retinitis pigmentosa. UNC119b regulates T-cell receptor signaling by modulating Lck kinase localization, linking it to immune dysfunction when dysregulated. Recombinant UNC119 proteins are engineered using bacterial or mammalian expression systems, often with affinity tags (e.g., GST, His-tag) for purification and experimental tracking.
Research applications include in vitro binding assays to map protein-lipid interactions, structural studies elucidating cargo recognition mechanisms, and therapeutic exploration for UNC119-associated pathologies. Notably, recombinant UNC119 serves as a molecular tool to investigate lipidated protein trafficking defects in neurological and immunological diseases. Recent studies also explore its potential as a drug target or delivery vehicle for gene therapies targeting retinal degeneration. Its dual roles in sensory and immune systems make it a unique model for studying compartmentalized signaling across cell types.
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