| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POU4F2 |
| Uniprot No | Q12837 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-265aa |
| 氨基酸序列 | MNTIPCTSAASSSSVPISHPSALAGTHHHHHHHHHHHHQPHQALEGELLEHLSPGLALGAMAGPDGAVVSTPAHAPHMATMNPMHQAALSMAHAHGLPSHMGCMSDVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGAEKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAGI |
| 预测分子量 | 36.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POU4F2重组蛋白的3篇代表性文献摘要：
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1. **文献名称**: *POU4F2/Brn-3b promotes cell proliferation and suppresses apoptosis in neuroblastoma and small cell lung carcinoma*
**作者**: Samson et al. (2008)
**摘要**: 研究发现POU4F2重组蛋白通过调控Bcl-2等凋亡相关基因的表达，促进神经母细胞瘤和小细胞肺癌的增殖，并抑制细胞凋亡，提示其在癌症治疗中的潜在靶点作用。
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2. **文献名称**: *Structural insights into POU4F2 DNA-binding specificity through recombinant protein analysis*
**作者**: Liang et al. (2019)
**摘要**: 通过重组蛋白的晶体结构分析，揭示了POU4F2与靶基因DNA结合域的特异性互作机制，关键氨基酸残基突变实验证实其转录调控功能的结构基础。
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3. **文献名称**: *POU4F2 is required for survival of spiral ganglion neurons and auditory function*
**作者**: Huang et al. (2010)
**摘要**: 利用重组蛋白体外模型，发现POU4F2对小鼠耳蜗螺旋神经节神经元的存活和听觉功能维持至关重要，敲除后导致听力损伤及神经元退化。
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4. **文献名称**: *Recombinant POU4F2 drives neuronal differentiation in pluripotent stem cells*
**作者**: Ninkina et al. (2014)
**摘要**: 研究显示，外源表达POU4F2重组蛋白可诱导多能干细胞向感觉神经元分化，为神经再生研究提供了体外实验工具。
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这些文献涵盖了POU4F2在癌症、听觉神经、结构生物学及干细胞分化中的功能研究。
POU4F2 (also known as BRN3B or BRN-3.2) is a transcription factor belonging to the POU protein family, characterized by a conserved DNA-binding domain called the POU domain. This domain consists of two subdomains—a POU-specific region and a homeodomain—that enable sequence-specific binding to DNA. POU4F2 plays critical roles in cellular differentiation, survival, and maintenance, particularly in neural and sensory systems. It is essential for the development of retinal ganglion cells, sensory neurons, and certain cranial ganglia, where it regulates genes involved in axon growth, synaptic connectivity, and apoptosis. Dysregulation of POU4F2 has been implicated in neurodevelopmental disorders and cancers, including neuroblastoma, melanoma, and breast cancer, where it may act as either an oncogene or tumor suppressor depending on cellular context.
Recombinant POU4F2 protein is engineered through molecular cloning techniques, typically expressed in bacterial, insect, or mammalian systems to ensure proper post-translational modifications. Its production enables functional studies, such as mapping DNA-binding motifs, analyzing protein-protein interactions (e.g., with co-regulators like SOX4), and investigating its role in gene regulatory networks. Researchers also use recombinant POU4F2 to screen small-molecule inhibitors or activators for therapeutic development. Structural studies using recombinant protein have revealed insights into how its POU domain interacts with DNA and epigenetic modifiers. Despite progress, challenges remain in understanding context-dependent regulatory mechanisms and tissue-specific isoforms. Current research focuses on its dual roles in neuroprotection and tumorigenesis, aiming to exploit POU4F2 pathways for regenerative medicine or targeted cancer therapies.
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