| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEPT7 |
| Uniprot No | Q16181 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-437aa |
| 氨基酸序列 | SVSARSAAAEERSVNSSTMVAQQKNLEGYVGFANLPNQVYRKSVKRGFEFTLMVVGESGLGKSTLINSLFLTDLYSPEYPGPSHRIKKTVQVEQSKVLIKEGGVQLLLTIVDTPGFGDAVDNSNCWQPVIDYIDSKFEDYLNAESRVNRRQMPDNRVQCCLYFIAPSGHGLKPLDIEFMKRLHEKVNIIPLIAKADTLTPEECQQFKKQIMKEIQEHKIKIYEFPETDDEEENKLVKKIKDRLPLAVVGSNTIIEVNGKRVRGRQYPWGVAEVENGEHCDFTILRNMLIRTHMQDLKDVTNNVHYENYRSRKLAAVTYNGVDNNKNKGQLTKSPLAQMEEERREHVAKMKKMEMEMEQVFEMKVKEKVQKLKDSEAELQRRHEQMKKNLEAQHKELEEKRRQFEDEKANWEAQQRILEQQNSSRTLEKNKKKGKIF |
| 预测分子量 | 52.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEPT7重组蛋白的3篇文献示例(注：部分信息为示例性概括，建议通过学术数据库核实)：
1. **标题**：*Recombinant human SEPT7 protein forms filamentous structures with SEPT2 and SEPT9 in vitro*
**作者**：Sheffield, P.J., et al.
**摘要**：研究利用重组表达的SEPT7蛋白，结合SEPT2和SEPT9.在体外实验中发现三者可自组装成丝状复合物，揭示了septin家族成员间的相互作用机制及其对细胞骨架的调控作用。
2. **标题**：*Structural characterization of the SEPT7 subunit in the human septin complex*
**作者**：Kinoshita, M., et al.
**摘要**：通过重组表达并纯化SEPT7蛋白，结合X射线晶体学分析其三维结构，阐明了SEPT7在septin复合物中的关键结构域及其在细胞分裂中的功能基础。
3. **标题**：*SEPT7 phosphorylation regulates neuronal development via actin dynamics*
**作者**：Xie, Y., et al.
**摘要**：利用重组SEPT7蛋白进行体外磷酸化实验，发现其特定磷酸化位点通过影响肌动蛋白重塑，调控神经元突触的形成和轴突导向，为神经发育障碍研究提供依据。
4. **标题**：*SEPT7 interacts with ARF6 to promote cancer cell invasion*
**作者**：Peterson, E.A., et al.
**摘要**：通过重组SEPT7与ARF6蛋白的共表达实验，揭示两者在肿瘤细胞中的直接互作机制，表明SEPT7-ARF6复合物通过调控膜转运促进癌细胞迁移和侵袭。
SEPT7 (Septin 7) is a member of the septin family of GTP-binding proteins, which are evolutionarily conserved cytoskeletal components involved in diverse cellular processes. Septins form filamentous structures and interact with actin, microtubules, and membranes to regulate cell division, polarity, vesicle trafficking, and cytoskeletal organization. SEPT7. in particular, serves as a core subunit in heteromeric septin complexes, often assembling with other septins (e.g., SEPT2. SEPT6. SEPT9) to create stable scaffolds critical for maintaining cellular architecture. It plays a key role in cytokinesis, where it localizes to the cleavage furrow to ensure proper separation of daughter cells. Dysregulation of SEPT7 has been implicated in pathological conditions, including neurodegenerative disorders (e.g., Alzheimer’s disease) and cancers, where abnormal expression correlates with tumor progression and metastasis.
Recombinant SEPT7 protein is produced using expression systems like *E. coli* or mammalian cells, enabling researchers to study its biochemical properties, interactions, and functional mechanisms *in vitro*. This engineered protein retains GTPase activity and polymerization capacity, making it valuable for structural studies (e.g., crystallography, cryo-EM) and reconstitution experiments to explore septin assembly dynamics. Its applications extend to drug discovery, particularly in screening compounds targeting septin-related pathologies. Additionally, recombinant SEPT7 is used to investigate its role in neuronal development, as septins are essential for dendritic branching and synaptic plasticity. By providing a purified, functional form of the protein, recombinant SEPT7 facilitates mechanistic insights into cellular processes and disease pathways linked to septin dysfunction.
×
