| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPA |
| Uniprot No | Q15257 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-358aa |
| 氨基酸序列 | AEGERQPPPDSSEEAPPATQNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFEYRVSEMWNEVHEEKEQAAKQSVSCDECIPLPRAGHCAPSEAIEKLVALLNTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG |
| 预测分子量 | 60.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPA重组蛋白的假设性参考文献示例(文献为虚构，供参考格式)：
1. **文献名称**: "Recombinant Expression and Functional Analysis of PTPA in PP2A Activation"
**作者**: Smith J. et al.
**摘要**: 研究通过大肠杆菌系统表达重组人源PTPA蛋白，证实其通过促进PP2A磷酸酶的构象变化增强酶活性，为体外研究PP2A调控机制提供工具。
2. **文献名称**: "Crystal Structure of PTPA Reveals Its Molecular Interaction with PP2A"
**作者**: Lee S. & Zhang Y.
**摘要**: 解析了重组PTPA蛋白的晶体结构，发现其C端结构域与PP2A的催化亚基特异性结合，揭示了激活PP2A的分子基础。
3. **文献名称**: "PTPA Knockdown and Recombinant Rescue in Cancer Cell Models"
**作者**: Garcia R. et al.
**摘要**: 利用siRNA敲低PTPA后，细胞增殖异常;外源添加重组PTPA蛋白可恢复PP2A功能并抑制肿瘤生长，提示其潜在治疗价值。
4. **文献名称**: "Role of Recombinant PTPA in Tau Protein Dephosphorylation"
**作者**: Müller T. et al.
**摘要**: 在阿尔茨海默病细胞模型中，重组PTPA通过激活PP2A促进Tau蛋白去磷酸化，为神经退行性疾病研究提供新思路。
(注：以上文献为模拟内容，实际研究需查阅真实数据库如PubMed。)
**Background of PTPA Recombinant Protein**
Protein tyrosine phosphatase activator (PTPA), also known as PPP2R4. is a conserved regulatory protein critical for the activation and functional modulation of protein phosphatase 2A (PP2A), one of the most abundant serine/threonine phosphatases in eukaryotes. PP2A plays a central role in cellular processes, including cell cycle regulation, signal transduction, and apoptosis, by dephosphorylating key substrates. PTPA acts as a specific chaperone that facilitates the correct folding and metal ion coordination required for PP2A’s catalytic activity, ensuring its functional maturation.
Recombinant PTPA protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to enable high-purity, scalable production for research and therapeutic applications. Its recombinant form retains the native structure and biochemical properties, allowing scientists to study PP2A regulation mechanisms, dissect signaling pathways, and explore disease-related dysregulation. Dysfunction of PP2A, often linked to aberrant PTPA activity, is implicated in cancers, neurodegenerative disorders (e.g., Alzheimer’s), and metabolic syndromes.
Research on PTPA recombinant protein has advanced drug discovery efforts, particularly in designing PP2A-targeted therapies. Additionally, it serves as a tool for structural studies, inhibitor screening, and understanding post-translational modifications that influence PP2A activity. Recent studies also highlight its potential role in viral infections, as some pathogens exploit PP2A pathways for replication.
Overall, PTPA recombinant protein is a vital reagent for unraveling PP2A’s complex biology and developing interventions for diseases tied to phosphatase dysregulation. Its utility spans basic research, translational medicine, and biopharmaceutical development.
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