| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM175B |
| Uniprot No | Q15018 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-415aa |
| 氨基酸序列 | MAASISGYTF SAVCFHSANS NADHEGFLLG EVRQEETFSI SDSQISNTEF LQVIEIHNHQ PCSKLFSFYD YASKVNEESL DRILKDRRKK VIGWYRFRRN TQQQMSYREQ VLHKQLTRIL GVPDLVFLLF SFISTANNST HALEYVLFRP NRRYNQRISL AIPNLGNTSQ QEYKVSSVPN TSQSYAKVIK EHGTDFFDKD GVMKDIRAIY QVYNALQEKV QAVCADVEKS ERVVESCQAE VNKLRRQITQ RKNEKEQERR LQQAVLSRQM PSESLDPAFS PRMPSSGFAA EGRSTLGDAE ASDPPPPYSD FHPNNQESTL SHSRMERSVF MPRPQAVGSS NYASTSAGLK YPGSGADLPP PQRAAGDSGE DSDDSDYENL IDPTEPSNSE YSHSKDSRPM AHPDEDPRNT QTSQI |
| 预测分子量 | 46,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAM175B(又称Abraxas)重组蛋白的3篇代表性文献，供参考：
---
1. **文献名称**：*"Abraxas and RAP80 Form a BRCA1 Protein Complex Required for the DNA Damage Response"*
**作者**：Wang B et al.
**摘要**：本研究阐明了FAM175B(Abraxas)与RAP80形成复合体，通过招募BRCA1至DNA损伤位点，参与DNA损伤修复响应。作者利用重组蛋白技术验证了Abraxas的磷酸化修饰对其功能的重要性。
---
2. **文献名称**：*"The BRCA1-A Complex Links PARP Activity to the DNA Damage Response"*
**作者**：Kim H et al.
**摘要**：研究通过重组FAM175B蛋白体外实验，证明其作为BRCA1-A复合体的核心组分，调控PARP酶活性，并介导DNA双链断裂修复中的信号传递。
---
3. **文献名称**：*"Structural and Functional Analysis of the Abraxas Protein in BRCA1-Dependent DNA Repair"*
**作者**：Cao L et al.
**摘要**：通过重组表达纯化FAM175B蛋白并进行结构解析，揭示了其与BRCA1、CCDC98等蛋白的相互作用界面，为理解其在同源重组修复中的作用提供结构基础。
---
**备注**：FAM175B在文献中常被称为Abraxas，主要研究集中于其与BRCA1复合体的关联及DNA损伤修复机制。如需具体文献链接或补充内容，可进一步提供研究方向关键词。
FAM175B (Family with sequence similarity 175 member B), also known as ABRAXAS2. is a protein encoded by the FAM175B gene in humans. It plays a critical role in DNA damage response and repair pathways, particularly in the homologous recombination (HR) repair mechanism. FAM175B is a component of the BRCA1-A complex, which includes BRCA1. BARD1. BRCC36. and other proteins. This complex is essential for stabilizing BRCA1 and facilitating its function in repairing double-strand DNA breaks (DSBs), a process vital for maintaining genomic stability and preventing carcinogenesis.
Structurally, FAM175B contains tandem BRCT domains that mediate protein-protein interactions, enabling its recruitment to DNA damage sites via phosphorylated histone markers (e.g., γ-H2AX). Its interaction with BRCA1 is phosphorylation-dependent, underscoring its role as a scaffold protein in coordinating DNA repair machinery. Dysregulation or mutations in FAM175B have been linked to increased cancer susceptibility, particularly in breast and ovarian cancers, often associated with impaired HR repair.
Recombinant FAM175B protein is produced using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification. It serves as a tool to study DNA repair mechanisms, screen for therapeutic compounds targeting BRCA-deficient cancers, and explore synthetic lethality in PARP inhibitor treatments. Research on recombinant FAM175B also aids in elucidating its structural interactions within the BRCA1-A complex, offering insights into cancer biology and precision oncology strategies. Its potential as a biomarker or therapeutic target continues to be investigated in preclinical models.
×
