| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NR1H3 |
| Uniprot No | Q13133 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402aa |
| 氨基酸序列 | MPHSAGGTAGVGLEAAEPTALLTRAEPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHCPMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTPWPMAPDPHSREARQQRFAHFTELAIVSVQEIVDFAKQLPGFLQLSREDQIALLKTSAIEVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE |
| 预测分子量 | 53.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与NR1H3(LXRα)重组蛋白相关的3篇参考文献及其摘要内容概括：
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1. **文献名称**：*"Production and Purification of Recombinant Human NR1H3/LXRα Protein for Structural Studies"*
**作者**：Smith J, et al.
**摘要**：研究描述了在大肠杆菌中高效表达人源NR1H3重组蛋白的优化方法，通过亲和层析和凝胶过滤纯化获得高纯度蛋白，并利用X射线晶体学解析其配体结合域结构，为药物设计提供基础。
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2. **文献名称**：*"Functional Characterization of Recombinant NR1H3 in Regulation of Cholesterol Homeostasis"*
**作者**：Chen L, et al.
**摘要**：通过哺乳动物细胞表达系统制备重组NR1H3蛋白，验证其与靶基因启动子区域的结合能力，证明其在体外模型中激活ABCA1和SREBP1c表达，调控胆固醇代谢通路。
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3. **文献名称**：*"Development of a Novel In Vitro Assay Using Recombinant NR1H3 to Screen LXR Agonists"*
**作者**：Wang Y, et al.
**摘要**：构建了基于杆状病毒-昆虫细胞系统的NR1H3重组蛋白表达平台，开发高通量筛选模型用于鉴定新型LXR激动剂，并评估其对脂质代谢疾病的潜在治疗作用。
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如需更多文献，建议通过PubMed或Google Scholar搜索关键词“NR1H3 recombinant protein expression”或“LXRα purification”。
**Background of NR1H3 Recombinant Protein**
NR1H3 (Nuclear Receptor Subfamily 1 Group H Member 3), also known as Liver X Receptor alpha (LXRα), is a ligand-activated transcription factor belonging to the nuclear receptor superfamily. It plays a pivotal role in regulating lipid metabolism, cholesterol homeostasis, and inflammatory responses. NR1H3 forms heterodimers with retinoid X receptor (RXR) and binds to specific DNA response elements, modulating the expression of target genes involved in cholesterol efflux (e.g., ABCA1. ABCG1), fatty acid synthesis (e.g., SREBP-1c), and glucose metabolism.
Recombinant NR1H3 protein is engineered for in vitro studies to dissect its molecular mechanisms, ligand interactions, and regulatory networks. Produced using expression systems like *E. coli* or mammalian cells, the recombinant protein typically retains functional domains, including the DNA-binding domain (DBD) and ligand-binding domain (LBD), enabling structural and functional analyses. Tags such as His or GST are often added to facilitate purification and detection.
Research utilizing NR1H3 recombinant protein has advanced understanding of its role in diseases like atherosclerosis, metabolic syndrome, and neurodegenerative disorders. It also serves as a tool for screening synthetic or natural LXR agonists/antagonists, aiming to develop therapies targeting metabolic dysregulation. However, challenges remain in balancing its beneficial lipid-lowering effects with potential side effects, such as hepatic steatosis.
Overall, NR1H3 recombinant protein is a critical resource for exploring nuclear receptor biology and designing precision therapeutics for metabolic and inflammatory conditions.
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