Recombinant Human ELAVL3 protein

中文名： ELAV样蛋白3(ELAVL3)重组蛋白
别名： ELAVL3；HUC；PLE21；ELAV-like protein 3

货号: PA2000-3043

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	ELAVL3
Uniprot No	Q14576
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-367aa
氨基酸序列	MVTQILGAMESQVGGGPAGPALPNGPLLGTNGATDDSKTNLIVNYLPQNMTQDEFKSLFGSIGDIESCKLVRDKITGQSLGYGFVNYSDPNDADKAINTLNGLKLQTKTIKVSYARPSSASIRDANLYVSGLPKTMSQKEMEQLFSQYGRIITSRILVDQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPLGAAEPITVKFANNPSQKTGQALLTHLYQSSARRYAGPLHHQTQRFRLDNLLNMAYGVKSPLSLIARFSPIAIDGMSGLAGVGLSGGAAGAGWCIFVYNLSPEADESVLWQLFGPFGAVTNVKVIRDFTTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGERVLQVSFKTSKQHKA
预测分子量	41.5 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ELAVL3重组蛋白的3条参考文献示例，包含文献名称、作者及摘要概括：

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1. **文献名称**：*"Recombinant ELAVL3 Protein Binds to AU-Rich Elements and Stabilizes Target mRNAs in Neuronal Cells"*

**作者**：Smith J, Lee T, García-Blanco MA

**摘要**：本研究成功构建并纯化了重组ELAVL3蛋白，通过电泳迁移率实验(EMSA)和RNA免疫沉淀(RIP)证实其特异性结合AU-rich RNA元件。进一步发现ELAVL3通过调控神经相关基因(如BDNF)的mRNA稳定性，影响神经元分化。

2. **文献名称**：*"Structural Insights into ELAVL3 RNA Recognition Motif Using X-ray Crystallography"*

**作者**：Brown K, Müller-McNicoll M, Darnell RB

**摘要**：首次解析了重组ELAVL3蛋白的RNA结合域晶体结构，揭示了其与RNA结合的分子机制。结构比对显示ELAVL3具有独特的疏水核心，可能解释其与家族其他成员(如ELAVL1/HuR)的功能差异。

3. **文献名称**：*"ELAVL3 Knockout Mice Exhibit Cerebellar Ataxia and Dysregulated Synaptic Protein Expression"*

**作者**：Lee S, Kim D, Ackerman SL

**摘要**：通过构建ELAVL3基因敲除小鼠模型，发现其小脑颗粒细胞发育异常并伴随共济失调。蛋白质组学分析表明，ELAVL3缺失导致突触相关蛋白的mRNA翻译效率下降，提示其在神经发育中的翻译调控作用。

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**备注**：上述文献为示例，实际研究中可查阅PubMed或Web of Science等数据库获取真实文献。ELAVL3的研究多聚焦于神经发育及RNA代谢，重组蛋白的制备常涉及原核/真核表达系统及功能验证。

背景信息

ELAVL3. also known as HuD, is a member of the Embryonic Lethal Abnormal Vision-like (ELAVL) family of RNA-binding proteins. These proteins are evolutionarily conserved and play critical roles in post-transcriptional gene regulation by binding to AU-rich elements (AREs) or other conserved motifs in the 3' untranslated regions (UTRs) of target mRNAs. ELAVL3 is predominantly expressed in neurons and neuroendocrine cells, where it stabilizes mRNAs, enhances their translation, and regulates RNA splicing or localization. It is particularly implicated in neuronal development, differentiation, and synaptic plasticity, with studies linking it to processes like axon guidance, neurite outgrowth, and long-term memory formation.

Recombinant ELAVL3 protein is engineered for in vitro studies to dissect its molecular interactions and functional mechanisms. Typically produced in bacterial (e.g., *E. coli*) or eukaryotic expression systems, the recombinant protein retains RNA-binding activity and can be purified using affinity tags (e.g., His-tag). Researchers utilize it to investigate RNA-protein binding kinetics, identify target transcripts via RNA immunoprecipitation (RIP) or CLIP-seq, and explore its role in neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s) or neurodevelopmental disorders. Dysregulation of ELAVL3 has been associated with conditions like paraneoplastic neurological syndromes, where autoantibodies against HuD are detected. Its recombinant form also serves as a tool for drug screening aimed at modulating RNA stability or neuroregeneration pathways. Structural studies using recombinant ELAVL3 have revealed conserved domains, including three RNA recognition motifs (RRMs) critical for its function. Overall, recombinant ELAVL3 is a vital resource for unraveling its neurobiological roles and therapeutic potential.