| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AIMP2 |
| Uniprot No | Q13155 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-320aa |
| 氨基酸序列 | MPMYQVKPYHGGGAPLRVELPTCMYRLPNVHGRSYGPAPGAGHVQEESNLSLQALESRQDDILKRLYELKAAVDGLSKMIQTPDADLDVTNIIQADEPTTLTTNALDLNSVLGKDYGALKDIVINANPASPPLSLLVLHRLLCEHFRVLSTVHTHSSVKSVPENLLKCFGEQNKKQPRQDYQLGFTLIWKNVPKTQMKFSIQTMCPIEGEGNIARFLFSLFGQKHNAVNATLIDSWVDIAIFQLKEGSSKEKAAVFRSMNSALGKSPWLAGNELTVADVVLWSVLQQIGGCSVTVPANVQRWMRSCENLAPFNTALKLLK |
| 预测分子量 | 39.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设存在的关于AIMP2重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
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1. **标题**: *"Expression and Structural Characterization of Recombinant AIMP2 in E. coli"*
**作者**: Kim JH, Lee SY, Park HS
**摘要**: 本研究利用大肠杆菌表达系统成功纯化重组人源AIMP2蛋白，并通过X射线晶体学解析其三维结构，揭示了其与tRNA合成酶复合物的结合位点，为后续功能研究奠定基础。
2. **标题**: *"AIMP2 Recombinant Protein Attenuates Neurodegeneration in Parkinson's Disease Models"*
**作者**: Chen L, Wang X, Zhang R
**摘要**: 实验表明，外源性重组AIMP2蛋白可通过抑制JNK信号通路减少多巴胺能神经元凋亡，在细胞和小鼠帕金森病模型中表现出神经保护作用，提示其潜在治疗价值。
3. **标题**: *"Proteomic Analysis of AIMP2-Interacting Partners Using Recombinant TAP-Tagged Protein"*
**作者**: Gupta S, Müller B, Schneider A
**摘要**: 通过构建串联亲和纯化(TAP)标签的重组AIMP2蛋白，鉴定了其在HeLa细胞中与p53、TGF-β通路蛋白的相互作用网络，揭示了AIMP2在肿瘤抑制中的新调控机制。
4. **标题**: *"Development of a High-Yield AIMP2 Recombinant Protein Production System for Therapeutic Screening"*
**作者**: Tanaka K, Ito T, Sato M
**摘要**: 优化昆虫细胞-杆状病毒表达系统，实现毫克级重组AIMP2蛋白的高效生产，并验证其体外抑制肺癌细胞迁移的活性，为药物筛选提供标准化蛋白样品。
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注：以上文献为模拟内容，实际研究中请通过PubMed/Google Scholar等平台检索真实发表的论文。
**Background of AIMP2 Recombinant Protein**
AIMP2 (Aminoacyl-tRNA Synthetase Complex Interacting Multifunctional Protein 2), also known as JTV-1 or SCYE1. is a component of the multi-tRNA synthetase complex (MSC), a critical machinery for protein synthesis. Initially identified as a scaffolding protein within the MSC, AIMP2 plays roles in stabilizing the complex and ensuring efficient translation. Beyond its canonical function, AIMP2 has emerged as a multifunctional regulator involved in diverse cellular processes, including cell proliferation, apoptosis, inflammation, and tumor suppression.
Notably, AIMP2 acts as a tumor suppressor by interacting with p53. enhancing its stability, and promoting apoptosis under stress conditions. Dysregulation or mutations in AIMP2 are linked to cancers, neurodegenerative diseases, and pulmonary fibrosis. For instance, its cleavage by caspases during apoptosis generates a pro-apoptotic fragment, while its interaction with TGF-β signaling components implicates it in fibrotic pathways.
Recombinant AIMP2 protein is produced via *in vitro* expression systems (e.g., *E. coli*, mammalian cells) to study its structure-function relationships, binding partners, and therapeutic potential. Its purified form enables biochemical assays, structural analyses (e.g., crystallography), and drug screening, particularly for cancer therapies targeting AIMP2-p53 interactions. Researchers also utilize recombinant AIMP2 to explore its role in inflammatory responses and as a biomarker in disease models.
Overall, AIMP2 recombinant protein serves as a vital tool for unraveling its dual roles in translation regulation and disease pathogenesis, offering insights for therapeutic development in oncology and fibrosis-related disorders.
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