| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XRCC4 |
| Uniprot No | Q13426 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-336aa |
| 氨基酸序列 | MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI |
| 预测分子量 | 65.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于XRCC4重组蛋白的3篇经典文献及其摘要概括：
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1. **文献名称**: *XRCC4 promotes DNA ligase IV translocation to sites of DNA damage*
**作者**: Critchlow, S.E., Jackson, S.P.
**摘要**: 该研究揭示了XRCC4在DNA双链断裂修复中的核心作用，通过与DNA连接酶IV(Lig4)形成稳定复合物，促进其向损伤位点募集，并调控非同源末端连接(NHEJ)的修复效率。
2. **文献名称**: *Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells*
**作者**: Grawunder, U., et al.
**摘要**: 实验证明XRCC4与DNA连接酶IV直接结合，显著增强其酶活性，并证实两者在V(D)J重组和NHEJ中的协同功能，为免疫系统多样性及基因组稳定性提供分子机制基础。
3. **文献名称**: *XRCC4 function in DNA nonhomologous end-joining: Interaction with the DNA ligase IV family member and analysis of mutations in radiosensitive human cells*
**作者**: Ahnesorg, P., Smith, P.
**摘要**: 通过结构域分析，明确了XRCC4的N端区域对结合DNA连接酶IV的关键性，并发现人类细胞中XRCC4突变会导致辐射敏感性增加，佐证其在DNA修复中的必要性。
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**备注**：上述文献均发表于2000年前后，集中于XRCC4的分子机制研究。如需近年研究(如XRCC4在癌症或CRISPR编辑中的应用)，建议补充具体方向以便进一步筛选。
**Background of XRCC4 Recombinant Protein**
XRCC4 (X-ray repair cross-complementing protein 4) is a critical DNA repair protein involved in the non-homologous end joining (NHEJ) pathway, the primary mechanism for repairing double-strand breaks (DSBs) in mammalian cells. It functions as a scaffold, stabilizing interactions between DNA ligase IV (LIG4) and other NHEJ factors, such as XLF (XRCC4-like factor), to facilitate the ligation of broken DNA ends. Structurally, XRCC4 contains an N-terminal domain for dimerization and a coiled-coil region that mediates interactions with LIG4 and regulatory factors like DNA-dependent protein kinase (DNA-PK).
Recombinant XRCC4 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) for biochemical and functional studies. Its purified form enables researchers to dissect NHEJ mechanisms, analyze protein-DNA interactions, and screen for inhibitors targeting DNA repair pathways. XRCC4’s role in maintaining genomic stability has broad implications, particularly in cancer biology, as NHEJ defects are linked to tumorigenesis and therapy resistance. Additionally, XRCC4-LIG4 complexes are essential for V(D)J recombination, a process critical for adaptive immunity.
Studies using recombinant XRCC4 have also advanced CRISPR-Cas9 genome-editing applications, where NHEJ efficiency influences editing outcomes. By modulating XRCC4 activity, researchers aim to improve precision in gene editing or sensitize cancer cells to radiation/chemotherapy. However, its overexpression in some cancers may promote repair of therapy-induced DSBs, highlighting its dual role as a therapeutic target. Overall, recombinant XRCC4 remains a vital tool for unraveling DNA repair dynamics and developing strategies to manipulate genomic integrity in research and medicine.
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