| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ME3 |
| Uniprot No | Q16798-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-590aa |
| 氨基酸序列 | RWTPTAPAQGCHSKPGPARPVPLKKRGYDVTRNPHLNKGMAFTLEERLQL GIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYR VLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLN SWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQC LPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGI NCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRIT KNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKG LIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTE QILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKS VTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQE VSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEA FVRSLVYTPDYDSFTLD |
| 预测分子量 | 67 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ME3(苹果酸酶3)重组蛋白研究的示例参考文献(内容为模拟示例，非真实文献)：
1. **《Structural and Functional Characterization of Recombinant Human ME3》**
- 作者：Smith, J. et al.
- 摘要：通过大肠杆菌表达系统纯化重组人源ME3蛋白，解析其晶体结构，揭示其NADP+结合域及催化机制，为代谢疾病研究提供结构基础。
2. **《ME3 Overexpression Promotes Tumor Growth via Enhanced Mitochondrial Metabolism》**
- 作者：Lee, H. & Zhang, Y.
- 摘要：研究重组ME3在结肠癌细胞中的过表达对三羧酸循环的调控作用，证实其通过增加α-酮戊二酸合成促进肿瘤增殖。
3. **《Optimization of ME3 Recombinant Protein Production in Yeast Systems》**
- 作者：Garcia, R. et al.
- 摘要：比较毕赤酵母和酿酒酵母表达ME3的产量差异，优化诱导条件使酶活性提高3倍，为工业化生产提供参考。
4. **《ME3 Deficiency Alters Lipid Metabolism in Mouse Models》**
- 作者：Wang, Q. et al.
- 摘要：利用重组ME3敲除小鼠模型，发现ME3缺失导致线粒体脂质代谢紊乱，并影响胰岛素敏感性。
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提示：实际文献需通过PubMed、Google Scholar等平台检索关键词“ME3 recombinant protein”“malic enzyme 3 expression”等获取。建议结合研究领域(如癌症、代谢疾病)进一步筛选。
**Background of ME3 Recombinant Protein**
Malic Enzyme 3 (ME3), a member of the malic enzyme family, is a mitochondrial NADP+-dependent enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate, generating NADPH and CO₂. This reaction links the tricarboxylic acid (TCA) cycle with cellular biosynthetic processes, playing a critical role in energy metabolism and redox balance. ME3 is distinct from its cytosolic counterpart, ME1. and the mitochondrial ME2. due to its unique kinetic properties, substrate preferences, and regulatory mechanisms.
The *ME3* gene, located on human chromosome 11. encodes a protein predominantly expressed in metabolically active tissues, including the liver, brain, and heart. Dysregulation of ME3 has been implicated in various pathologies, such as cancer, neurodegenerative disorders, and metabolic syndromes. For instance, ME3 overexpression in certain cancers supports tumor growth by enhancing NADPH production, which sustains antioxidant defenses and macromolecule synthesis.
Recombinant ME3 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional enzyme for research and therapeutic applications. Its production enables detailed studies of ME3’s structure, catalytic mechanisms, and interactions with inhibitors or modulators. Additionally, recombinant ME3 serves as a tool to investigate metabolic reprogramming in diseases and explore targeted therapies.
Despite progress, ME3’s full physiological and pathological significance remains understudied compared to other malic enzymes. Challenges include its low natural abundance, structural complexity, and overlapping functions with ME2. Ongoing research aims to elucidate ME3-specific regulatory networks and validate its potential as a biomarker or drug target in precision medicine.
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