| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | MPK5 |
| Uniprot No | Q10N20 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-369aa |
| 氨基酸序列 | MDGAPVAEFRPTMTHGGRYLLYDIFGNKFEVTNKYQPPIMPIGRGAYGIVCSVMNFETREMVAIKKIANAFNNDMDAKRTLREIKLLRHLDHENIIGIRDVIPPPIPQAFNDVYIATELMDTDLHHIIRSNQELSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARPSSESDMMTEYVVTRWYRAPELLLNSTDYSAAIDVWSVGCIFMELINRQPLFPGRDHMHQMRLITEVIGTPTDDELGFIRNEDARKYMRHLPQYPRRTFASMFPRVQPAALDLIERMLTFNPLQRITVEEALDHPYLERLHDIADEPICLEPFSFDFEQKALNEDQMKQLIFNEAIEMNPNIRY |
| 预测分子量 | 48.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MPK5重组蛋白的参考文献示例(注：部分内容为假设性概括，实际文献需进一步检索验证)：
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1. **文献名称**: "Recombinant expression and functional characterization of Arabidopsis MPK5 in plant immunity"
**作者**: Zhang, X., et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了拟南芥MPK5重组蛋白，通过体外激酶实验证实其磷酸化下游底物WRKY转录因子的能力，揭示了MPK5在植物抗病信号通路中的关键作用。
2. **文献名称**: "Optimization of MPK5 protein purification for structural analysis"
**作者**: Lee, S., et al.
**摘要**: 通过优化表达系统和纯化条件(如亲和层析和凝胶过滤)，获得了高纯度、高活性的重组MPK5蛋白，为其晶体结构解析及激酶机制研究奠定基础。
3. **文献名称**: "MPK5 interacts with MKK4/MKK5 in the MAPK cascade: Evidence from in vitro reconstitution assays"
**作者**: Wang, Y., et al.
**摘要**: 利用重组MPK5蛋白与上游激酶MKK4/MKK5进行体外重组实验，证实MPK5可被MKK4/5特异性激活，并参与ABA(脱落酸)信号转导通路。
4. **文献名称**: "A high-throughput screening platform using recombinant MPK5 for kinase inhibitor discovery"
**作者**: Chen, L., et al.
**摘要**: 建立基于重组MPK5蛋白的高通量筛选模型，用于鉴定新型激酶抑制剂，为开发靶向MAPK信号通路的药物提供工具。
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**备注**：以上文献名称及内容为示例性质，实际研究中需通过学术数据库(如PubMed、Web of Science)检索具体文献。若需真实文献，建议以“MPK5 recombinant protein”、“AtMPK5 expression”等关键词进一步查询。
**Background of MPK5 Recombinant Protein**
MPK5 (Mitogen-Activated Protein Kinase 5) is a member of the evolutionarily conserved MAPK family, which plays a central role in intracellular signal transduction pathways. These kinases regulate cellular responses to external stimuli, including stress, hormones, and growth factors, by phosphorylating downstream targets. In plants, MPK5 homologs are implicated in defense signaling, abiotic stress tolerance (e.g., drought, salinity), and developmental processes. For instance, studies in model organisms like *Arabidopsis thaliana* have shown that MPK5 activation is linked to pathogen-associated molecular pattern (PAMP)-triggered immunity and jasmonic acid signaling.
Recombinant MPK5 protein is produced using genetic engineering techniques, often expressed in heterologous systems like *E. coli* or insect cells to ensure high yield and purity. The recombinant form retains the kinase activity of native MPK5. enabling in vitro studies to dissect its enzymatic properties, substrate specificity, and interaction networks. Researchers utilize tools like phosphorylation assays, structural analysis (e.g., X-ray crystallography), and inhibitor screening to explore its functional mechanisms.
The development of MPK5 recombinant protein has advanced both basic and applied research. It serves as a critical tool for elucidating MAPK signaling cascades in plants and their roles in stress adaptation, potentially informing crop improvement strategies. Additionally, it aids in drug discovery, particularly in identifying kinase inhibitors for therapeutic or agricultural applications. However, challenges remain in optimizing expression conditions, maintaining post-translational modifications, and ensuring biological relevance in vitro. Ongoing research continues to refine MPK5 recombinant protein applications, bridging molecular insights with biotechnological innovation.
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