| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | csrA |
| Uniprot No | B1XCM4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-61aa |
| 氨基酸序列 | MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHREEIYQRIQAEKSQQSSY |
| 预测分子量 | 6.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **《Regulation of virulence factors by CsrA in Escherichia coli》**
- **作者**: J. L. Romeo
- **摘要**: 探讨CsrA蛋白在大肠杆菌中通过结合靶标mRNA调控毒力因子表达的机制，分析重组CsrA在体外对生物膜形成和运动性的影响。
2. **《Structural and functional analysis of the CsrA protein from Pseudomonas aeruginosa》**
- **作者**: M. Thompson et al.
- **摘要**: 研究铜绿假单胞菌CsrA重组蛋白的晶体结构，揭示其RNA结合域的关键位点，并验证其通过调控代谢相关基因参与抗生素耐受性。
3. **《CsrA-mediated post-transcriptional regulation in Salmonella typhimurium》**
- **作者**: K. S. Baker, R. G. Hernandez
- **摘要**: 利用重组CsrA蛋白研究其在沙门氏菌中通过结合hilD mRNA调控宿主细胞内生存能力的分子通路，揭示其在感染过程中的作用。
4. **《High-yield expression and purification of recombinant CsrA for biotechnological applications》**
- **作者**: T. Müller et al.
- **摘要**: 报道一种高效的大肠杆菌表达系统，优化重组CsrA蛋白的纯化流程，验证其稳定性及在合成生物学工具开发中的潜在应用。
**Background of CsrA Recombinant Protein**
The CsrA (Carbon storage regulator A) protein is a post-transcriptional global regulator in bacteria, first characterized in *Escherichia coli*. It belongs to the RsmA (Regulator of secondary metabolism) family of RNA-binding proteins, playing a pivotal role in bacterial adaptation to environmental changes by controlling gene expression related to carbon metabolism, biofilm formation, motility, and virulence. CsrA typically functions by binding to the 5'-untranslated region (UTR) or initial coding sequences of target mRNAs, altering their stability or translation efficiency. This regulation is often counteracted by small non-coding RNAs (e.g., CsrB/CsrC in *E. coli*), which sequester CsrA to fine-tune its activity.
Recombinant CsrA protein is produced via genetic engineering, often expressed in *E. coli* using plasmid vectors with inducible promoters (e.g., T7 or lac). The protein is purified using affinity chromatography, leveraging tags like His-tag or GST for efficient isolation. Structural studies (e.g., X-ray crystallography) reveal CsrA forms a symmetrical homodimer, with each monomer containing a β-strand-rich fold and RNA-binding surfaces.
Research on recombinant CsrA focuses on elucidating its regulatory mechanisms, interactions with RNA or partner proteins, and its role in bacterial pathogenesis. It serves as a model for understanding post-transcriptional regulation and is explored as a potential therapeutic target to disrupt bacterial virulence or antibiotic resistance. Challenges in its application include maintaining native conformation during purification and addressing solubility issues. Overall, CsrA recombinant protein remains a critical tool for dissecting bacterial regulatory networks and developing antimicrobial strategies.
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