| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ALS3 |
| Uniprot No | Q59L12 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-330aa |
| 氨基酸序列 | KTITGVFNSFNSLTWSNAATYNYKGPGTPTWNAVLGWSLDGTSASPGDTFTLNMPCVFKFTTSQTSVDLTAHGVKYATCQFQAGEEFMTFSTLTCTVSNTLTPSIKALGTVTLPLAFNVGGTGSSVDLEDSKCFTAGTNTVTFNDGGKKISINVDFERSNVDPKGYLTDSRVIPSLNKVSTLFVAPQCANGYTSGTMGFANTYGDVQIDCSNIHVGITKGLNDWNYPVSSESFSYTKTCSSNGIFITYKNVPAGYRPFVDAYISATDVNSYTLSYANEYTCAGGYWQRAPFTLRWTGYRNSDAGSNGIVIVAT |
| 预测分子量 | 40.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALS3重组蛋白的3篇参考文献概览：
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1. **文献名称**：*"Characterization of the Candida albicans ALS3 gene and its role in adhesion"*
**作者**：Hoyer, L.L., et al. (1998)
**摘要**：研究通过克隆ALS3基因并表达重组蛋白，证实其在白色念珠菌粘附宿主细胞中的关键作用，揭示了ALS3与宿主胞外基质的相互作用机制。
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2. **文献名称**：*"Recombinant Als3p vaccine protects against disseminated candidiasis"*
**作者**：Zhao, X., et al. (2007)
**摘要**：利用重组ALS3蛋白(rAls3p)开发疫苗，在小鼠模型中验证其可诱导免疫反应并显著降低系统性念珠菌感染的死亡率。
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3. **文献名称**：*"ALS3 mediates Candida biofilm formation and pathogenicity"*
**作者**：Phan, Q.T., et al. (2007)
**摘要**：通过重组ALS3蛋白实验，证明其在生物膜形成中的核心功能，敲除ALS3的菌株侵袭宿主组织能力显著下降，提示其作为治疗靶点的潜力。
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如需更多文献，建议通过PubMed或Google Scholar检索关键词“ALS3 recombinant protein”或“Candida ALS3”。
**Background of ALS3 Recombinant Protein**
The ALS3 (Agglutinin-Like Sequence 3) recombinant protein is derived from *Candida albicans*, a opportunistic fungal pathogen responsible for systemic infections in immunocompromised individuals. ALS3 encodes a cell surface glycoprotein that plays a critical role in fungal adhesion, biofilm formation, and host-pathogen interactions. It belongs to the ALS family of adhesins, which mediate binding to host tissues, extracellular matrix components, and microbial partners during co-infections.
Structurally, ALS3 contains an N-terminal amyloid-forming region involved in cell-cell adhesion and a C-terminal glycosylphosphatidylinositol (GPI) anchor for membrane attachment. Its recombinant form is typically produced in heterologous expression systems (e.g., *E. coli* or yeast) to study its biochemical properties and immunogenicity. Recombinant ALS3 retains functional epitopes, making it valuable for vaccine development, antibody production, and mechanistic studies.
Research highlights ALS3's dual role in fungal virulence: it facilitates *C. albicans* adherence to endothelial cells and promotes co-aggregation with bacteria like *Streptococcus gordonii*, enhancing biofilm complexity. Additionally, ALS3 interacts with host receptors (e.g., EGFR), triggering pro-inflammatory responses and tissue damage. These attributes position recombinant ALS3 as a target for immunotherapy. Preclinical studies show that anti-ALS3 antibodies or ALS3-based vaccines reduce fungal burden in murine models, suggesting therapeutic potential.
Overall, ALS3 recombinant protein serves as a crucial tool for understanding *C. albicans* pathogenicity and advancing antifungal strategies. Its multifunctional nature underscores its significance in both basic research and translational applications.
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