| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | UGT71C1 |
| Uniprot No | O82381 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-481aa |
| 氨基酸序列 | MGKQEDAELVIIPFPFSGHILATIELAKRLISQDNPRIHTITILYWGLPFIPQADTIAFLRSLVKNEPRIRLVTLPEVQDPPPMELFVEFAESYILEYVKKMVPIIREALSTLLSSRDESGSVRVAGLVLDFFCVPMIDVGNEFNLPSYIFLTCSAGFLGMMKYLPERHREIKSEFNRSFNEELNLIPGYVNSVPTKVLPSGLFMKETYEPWVELAERFPEAKGILVNSYTALEPNGFKYFDRCPDNYPTIYPIGPILCSNDRPNLDSSERDRIITWLDDQPESSVVFLCFGSLKNLSATQINEIAQALEIVDCKFIWSFRTNPKEYASPYEALPHGFMDRVMDQGIVCGWAPQVEILAHKAVGGFVSHCGWNSILESLGFGVPIATWPMYAEQQLNAFTMVKELGLALEMRLDYVSEDGDIVKADEIAGTVRSLMDGVDVPKSKVKEIAEAGKEAVDGGSSFLAVKRFIGDLIDGVSISK |
| 预测分子量 | 56.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UGT71C1重组蛋白的参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: *Functional characterization of recombinant UGT71C1 from Arabidopsis thaliana: a key enzyme in flavonoid glycosylation*
**作者**: Smith A, Jones B
**摘要**: 本研究在大肠杆菌中重组表达了拟南芥来源的UGT71C1蛋白，并分析其催化活性。结果表明，UGT71C1可特异性催化类黄酮的糖基化反应，对槲皮素和山柰酚表现出高底物亲和力，揭示了其在植物次级代谢中的关键作用。
2. **文献名称**: *Heterologous expression and structural analysis of UGT71C1: insights into substrate recognition*
**作者**: Chen L, et al.
**摘要**: 通过酵母系统表达并纯化UGT71C1重组蛋白，结合晶体结构解析，发现其活性口袋的关键氨基酸残基参与底物结合，为酶工程改造提供了结构基础。
3. **文献名称**: *UGT71C1-mediated glycosylation enhances the stability of phenolic compounds in vitro*
**作者**: Wang X, et al.
**摘要**: 利用重组UGT71C1进行体外酶促反应，证明其糖基化修饰可显著提高多酚化合物的热稳定性和水溶性，提示其在工业生物催化中的应用潜力。
4. **文献名称**: *Role of UGT71C1 in plant stress response: overexpression in Nicotiana benthamiana*
**作者**: Kim H, et al.
**摘要**: 在本氏烟草中过表达重组UGT71C1.发现其通过调控活性氧相关代谢物的糖基化，增强植物对氧化胁迫的抗性，为抗逆作物开发提供新思路。
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建议通过PubMed、Web of Science或Google Scholar以“UGT71C1 recombinant protein”为关键词检索最新文献，以获取具体研究数据。
UGT71C1 is a plant-derived uridine diphosphate (UDP)-dependent glycosyltransferase belonging to the GT1 family, which catalyzes the transfer of sugar moieties from activated nucleotide sugars to diverse small molecule acceptors. Initially identified in *Arabidopsis thaliana*, this enzyme plays a critical role in secondary metabolism by glycosylating flavonoids, phenylpropanoids, and other phenolic compounds, enhancing their stability, solubility, and bioactivity. Its activity is pivotal in plant defense mechanisms against biotic and abiotic stresses, as glycosylation often modifies the reactivity or compartmentalization of phytochemicals.
Recombinant UGT71C1 protein is produced through heterologous expression systems, typically in *E. coli* or yeast, enabling large-scale purification for functional and structural studies. The recombinant form retains enzymatic specificity, allowing researchers to explore its substrate promiscuity, kinetic parameters, and catalytic mechanisms. Structural analyses reveal a conserved GT-B fold with two Rossmann-like domains, where the N-terminal domain binds the acceptor molecule, and the C-terminal domain interacts with the UDP-sugar donor.
Biotechnologically, UGT71C1 has garnered interest for its potential in synthesizing glycosylated natural products, which are valuable in pharmaceuticals, nutraceuticals, and agricultural chemicals. For example, it can glycosylate resveratrol or quercetin to improve their bioavailability. Additionally, engineered variants of UGT71C1 are being explored to expand substrate range or enhance catalytic efficiency, leveraging directed evolution or rational design. Studies on this enzyme also contribute to understanding the evolution of plant metabolic pathways and their adaptive significance. Overall, UGT71C1 exemplifies the intersection of plant biochemistry and applied enzymology, offering tools for sustainable production of high-value glycosides.
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