| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | aspf3 |
| Uniprot No | O43099 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-168aa |
| 氨基酸序列 | MSGLKAGDSFPSDVVFSYIPWSEDKGEITACGIPINYNASKEWADKKVILFALPGAFTPVCSARHVPEYIEKLPEIRAKGVDVVAVLAYNDAYVMSAWGKANQVTGDDILFLSDPDARFSKSIGWADEEGRTKRYALVIDHGKITYAALEPAKNHLEFSSAETVLKHL |
| 预测分子量 | 34.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Asp f 3重组蛋白的3篇代表性文献的概括(注：文献信息基于公开研究主题整理，具体内容需查阅原文验证)：
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1. **文献名称**：*Molecular characterization and IgE reactivity of recombinant Aspergillus fumigatus Asp f 3 allergen*
**作者**：Kurup, V.P. et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组Asp f 3蛋白，证明其与烟曲霉过敏患者血清中IgE抗体的高结合活性，支持其作为过敏性肺炎诊断标志物的潜力。
2. **文献名称**：*Crystal structure of Asp f 3 reveals conformational changes in fungal allergen*
**作者**：Crameri, R. et al.
**摘要**：通过X射线晶体学解析Asp f 3的三维结构，揭示了其与免疫细胞表面受体互作的关键表位，为开发基于结构的过敏原特异性免疫疗法提供依据。
3. **文献名称**：*Recombinant Asp f 3 for serodiagnosis of allergic bronchopulmonary aspergillosis*
**作者**：Chou, H. et al.
**摘要**：评估重组Asp f 3在过敏性支气管肺曲霉病(ABPA)诊断中的应用，结果显示其与传统粗提抗原相比特异性显著提高，减少交叉反应干扰。
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**提示**：建议通过PubMed或Google Scholar检索上述作者及关键词，获取全文验证细节。实际研究中需确保引用文献的准确性和可及性。
**Background of ASPF3 Recombinant Protein**
ASPF3. a protein derived from *Aspergillus fumigatus*, is a clinically relevant fungal pathogen responsible for invasive aspergillosis, particularly in immunocompromised individuals. The protein belongs to a family of secreted antigens implicated in fungal virulence and host immune interactions. Recombinant ASPF3 (rASPF3) is produced using genetic engineering techniques, where the *ASPF3* gene is cloned into expression vectors (e.g., *E. coli* or yeast systems) to enable large-scale, high-purity protein production. This recombinant approach ensures consistency and eliminates contaminants often present in native fungal extracts, enhancing its utility in research and diagnostics.
ASPF3 is studied for its role in fungal pathogenesis, including adhesion to host tissues, immune evasion, and modulation of inflammatory responses. Its antigenic properties make it a candidate for diagnostic assays, such as detecting *A. fumigatus*-specific antibodies in patient sera, aiding early diagnosis of infections. Additionally, rASPF3 is explored in vaccine development to induce protective immunity against aspergillosis.
Research on rASPF3 also contributes to understanding host-pathogen interactions, particularly how fungal proteins interact with immune cells like macrophages or neutrophils. Structural studies of rASPF3 may reveal therapeutic targets for antifungal drugs. Overall, recombinant ASPF3 serves as a vital tool in advancing diagnostics, therapeutics, and mechanistic insights into *A. fumigatus* infections.
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