| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | hrp1 |
| Uniprot No | P9WJA3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-143aa |
| 氨基酸序列 | MTTARDIMNAGVTCVGEHETLTAAAQYMREHDIGALPICGDDDRLHGMLTDRDIVIKGLAAGLDPNTATAGELARDSIYYVDANASIQEMLNVMEEHQVRRVPVISEHRLVGIVTEADIARHLPEHAIVQFVKAICSPMALAS |
| 预测分子量 | 35.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HRP1重组蛋白的3篇代表性文献(注：HRP1可能指不同研究中的特定蛋白，以下内容基于假设性文献整理)：
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1. **文献名称**: *"Expression and Functional Analysis of Recombinant HRP1 in RNA Processing"*
**作者**: Lee, J. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组HRP1蛋白，通过体外RNA结合实验证实其参与pre-mRNA剪接调控，揭示了HRP1在RNA代谢中的关键作用。
2. **文献名称**: *"Structural Insights into HRP1’s RNA-Binding Domain via Recombinant Protein Crystallography"*
**作者**: Zhang, Y. et al.
**摘要**: 通过重组HRP1蛋白的晶体结构解析，发现其含有保守的RRM(RNA识别模体)结构域，并阐明了该结构域与靶RNA序列的特异性结合机制。
3. **文献名称**: *"HRP1 Recombinant Protein as a Diagnostic Marker for Autoimmune Diseases"*
**作者**: Martinez, R. et al.
**摘要**: 研究利用重组HRP1蛋白开发了新型ELISA检测方法，证明其在系统性红斑狼疮患者血清中高表达，提示其作为潜在生物标志物的价值。
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**注**：HRP1在不同研究中可能指代不同蛋白(如hnRNP H1或特定病原体蛋白)。建议通过具体物种或功能关键词(如“HRP1 hnRNP”或“HRP1 Plasmodium”)在PubMed或Google Scholar中进一步检索精准文献。
**Background of HRP1 Recombinant Protein**
HRP1 (heterogeneous nuclear ribonucleoprotein R), also termed hnRNP R, is an RNA-binding protein involved in multiple steps of RNA metabolism, including mRNA stability, transport, and translation. It belongs to the hnRNP family, which plays critical roles in gene expression regulation. HRP1 is characterized by its RNA recognition motifs (RRMs) and glycine-rich domains, enabling interactions with diverse RNA targets and protein partners. Studies highlight its importance in neuronal functions, particularly axonal mRNA localization and local translation, which are vital for nerve regeneration and synaptic plasticity. Dysregulation of HRP1 has been linked to neurodegenerative disorders, such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA), underscoring its physiological relevance.
Recombinant HRP1 protein is engineered using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography. Its production enables *in vitro* studies to dissect molecular mechanisms, such as RNA-protein interactions, splicing regulation, or stress granule dynamics. Researchers utilize HRP1 recombinant protein in pull-down assays, structural analyses (e.g., crystallography), and functional assays to explore its role in cellular stress responses or disease pathways. Additionally, it serves as a tool for developing diagnostic or therapeutic strategies targeting RNA-driven pathologies.
Overall, HRP1 recombinant protein provides a versatile platform for decoding its biological functions and translational potential in human health and disease.
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