| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | mgsA |
| Uniprot No | P09341 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-107aa |
| 氨基酸序列 | ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN |
| 预测分子量 | 7.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于mgsA重组蛋白的3-4条示例参考文献(内容为虚构示例，仅用于演示格式)：
1. **文献名称**：*Cloning and Expression of Recombinant mgsA in Escherichia coli for Methylglyoxal Synthesis*
**作者**：Smith J. et al.
**摘要**：本研究成功将mgsA基因克隆至大肠杆菌表达系统，纯化获得高活性重组MgsA蛋白，并验证其催化丙酮酸生成甲基乙二醛的功能，为后续代谢研究提供工具。
2. **文献名称**：*Functional Characterization of Recombinant mgsA in Bacterial Stress Response*
**作者**：Lee S. et al.
**摘要**：通过异源表达mgsA重组蛋白，揭示了其在细菌酸应激反应中调控甲基乙二醛代谢通路的机制，证明其与生物膜形成的关联性。
3. **文献名称**：*Optimization of mgsA Recombinant Protein Production Using a High-Density Fermentation System*
**作者**：Zhang Y. et al.
**摘要**：开发了一种高效发酵工艺，显著提升mgsA重组蛋白的产量，并通过质谱和酶动力学分析确认其结构与功能完整性。
4. **文献名称**：*Structural Insights into mgsA Catalytic Mechanism via Crystallography*
**作者**：Chen L. et al.
**摘要**：解析了重组MgsA蛋白的晶体结构，阐明其活性位点关键氨基酸残基在底物结合与催化中的作用，为定向改造提供理论依据。
(注：以上文献为模拟示例，实际引用需检索真实数据库如PubMed、Web of Science等获取。)
**Background of MgsA Recombinant Protein**
MgsA (methylglyoxal synthase A) is a key enzyme involved in the methylglyoxal (MG) pathway, a metabolic bypass that operates under glycolytic stress in bacteria such as *Escherichia coli*. It catalyzes the conversion of dihydroxyacetone phosphate (DHAP), a glycolytic intermediate, into methylglyoxal, a highly reactive α-oxoaldehyde. This pathway is typically activated under conditions of excess sugar influx or metabolic imbalance, serving as a detoxification mechanism to mitigate the accumulation of toxic phosphorylated intermediates.
The *mgsA* gene encoding this enzyme has been extensively studied for its role in bacterial stress response and its connection to advanced glycation end-product (AGE) formation, which is linked to cellular damage in eukaryotes. Recombinant MgsA protein is produced through heterologous expression systems, such as *E. coli* or yeast, by cloning the *mgsA* gene into expression vectors under inducible promoters. This allows large-scale production and purification for biochemical and structural studies.
Research on MgsA has provided insights into microbial metabolism under stress, potential targets for antimicrobial strategies, and the molecular basis of MG-related cytotoxicity. Additionally, recombinant MgsA is utilized in industrial applications, including enzymatic synthesis of chiral compounds and studies on glycation processes relevant to diabetes and aging. Despite its simplicity, the enzyme's regulation and interaction with cellular components remain areas of active investigation, highlighting its importance in both basic and applied microbiology.
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