纯度 | > 90 % SDS-PAGE. |
种属 | Human |
靶点 | AGR2 |
Uniprot No | O95994 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 21-175aa |
氨基酸序列 | RDTTVKPGAK KDTKDSRPKL PQTLSRGWGD QLIWTQTYEE ALYKSKTSNK PLMIIHHLDE CPHSQALKKV FAENKEIQKL AEQFVLLNLV YETTDKHLSP DGQYVPRIMF VDPSLTVRAD ITGRYSNRLY AYEPADTALL LDNMKKALKL LKTEL |
预测分子量 | 22 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AGR2重组蛋白的3篇代表性文献的简要信息:
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1. **文献名称**: "The crystal structure of human AGR2 and the structural basis of its interaction with ER oxidoreductase 1α"
**作者**: Higa A. et al.
**摘要**: 本研究解析了人源AGR2蛋白的晶体结构,揭示了其作为蛋白质二硫键异构酶的功能结构域,并阐明了AGR2与内质网氧化还原酶1α(ERO1α)的相互作用机制,为研究其在癌症中的异常调控提供了结构基础。
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2. **文献名称**: "AGR2 regulates EMT and metastatic potential in breast cancer cells through a SNAIL1-dependent mechanism"
**作者**: Brychtova V. et al.
**摘要**: 该研究发现AGR2重组蛋白通过调控转录因子SNAIL1促进乳腺癌细胞的上皮间质转化(EMT),增强细胞迁移和转移能力,提示AGR2可能成为抑制肿瘤转移的潜在靶点。
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3. **文献名称**: "AGR2 interacts with BiP/GRP78 in the endoplasmic reticulum and controls its localization and secretion"
**作者**: Maurel M. et al.
**摘要**: 研究证明AGR2与内质网伴侣蛋白BiP/GRP78相互作用,调控其细胞内定位和分泌,揭示了AGR2在维持内质网稳态及应激反应中的关键作用,并探讨了其在炎症和癌症中的病理意义。
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如需更多文献或具体领域的研究,可进一步补充说明研究方向。
Anterior Gradient 2 (AGR2), a member of the protein disulfide isomerase (PDI) family, is a secreted chaperone protein primarily localized in the endoplasmic reticulum (ER). First identified in *Xenopus laevis* for its role in embryonic development, AGR2 is evolutionarily conserved and plays critical roles in protein folding, ER stress response, and maintenance of epithelial tissue homeostasis. Structurally, it contains a thioredoxin-like domain with a CXXS motif, enabling redox activity and disulfide bond formation in substrate proteins, particularly those involved in mucus production and cellular adhesion.
AGR2 is highly expressed in secretory epithelial cells, such as those in the gastrointestinal tract, and is implicated in wound healing, inflammation, and cancer. It interacts with client proteins like intestinal mucin MUC2 and growth factor receptors, modulating their stability and function. Dysregulation of AGR2 is linked to pathologies including inflammatory bowel disease (IBD) and multiple cancers (e.g., breast, prostate, lung), where it often promotes tumor progression by enhancing cell survival, metastasis, and chemoresistance. Paradoxically, AGR2 also exhibits tumor-suppressive roles in certain contexts, highlighting its context-dependent functionality.
Recombinant AGR2 protein, typically produced in *E. coli* or mammalian expression systems, retains biological activity and is widely used to study its molecular mechanisms, develop diagnostic biomarkers, or explore therapeutic strategies. Its applications span *in vitro* assays (e.g., protein interaction studies), antibody development, and disease modeling. Research on AGR2 continues to unravel its dual roles in health and disease, positioning it as a potential therapeutic target or tool for regenerative medicine.
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