| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | uvsE |
| Uniprot No | B7IR00 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MIMRFGYVSHAMALWDCSPAKTMTFTSFKKLSKQEREDKLYHVIRQNLEHTIRILHYNIAHEIPLYRLSSSIVPLATHPEVEFDYIGVFTPLWRKIGALIKEHNLRISFHPNQFTLFTSDKPHITTNAITDMTYHYKILDAIGIADSSYINIHVGGAYGNKEKAIERFHENIKKLPAHIKKQMTLENDDKTYTTSETLSICQKENIPFVFDYHHHMANLCEQPLEELLPAIFETWSHTNISPKVHISSPRSEKEFRAHAEYIDLEFIKPFLHVAKKNNHNFDIMIESKQKDLALFRLIDELSAIRGIKRISGAMLQW |
| 预测分子量 | 44.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于UvsE重组蛋白的参考文献示例(内容基于学术文献常见主题概括，具体文献需进一步验证)：
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1. **文献名称**：*"Characterization of UvsE Recombinant Protein from Bacteriophage T4 and Its Role in DNA Repair"*
**作者**：Smith A. et al.
**摘要**：研究从T4噬菌体中克隆并表达UvsE重组蛋白，验证其结合紫外损伤DNA的能力，揭示其在错配修复和紫外线抗性中的功能。
2. **文献名称**：*"Structural and Functional Analysis of UvsE Endonuclease in Microbial DNA Damage Response"*
**作者**：Johnson R.B. et al.
**摘要**：解析UvsE重组蛋白的晶体结构，证明其作为内切酶特异性切割紫外诱导的嘧啶二聚体，提出其在基因编辑工具开发中的潜力。
3. **文献名称**：*"Application of UvsE Protein in Biosensors for Rapid Detection of DNA Damage"*
**作者**：Lee C. et al.
**摘要**：利用重组UvsE蛋白与损伤DNA结合的特性，开发新型荧光生物传感器，用于环境毒素或辐射暴露的快速检测。
4. **文献名称**：*"Optimization of UvsE Recombinant Expression in E. coli for High-Throughput Screening"*
**作者**：Chu W. et al.
**摘要**：通过优化大肠杆菌表达系统提高UvsE重组蛋白产量，并建立基于其活性的高通量筛选平台，用于抗紫外线化合物筛选。
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(注：以上文献标题和摘要为模拟概括，实际文献需通过PubMed、Web of Science等平台检索确认。)
UvsE recombinant protein is a genetically engineered enzyme derived from bacteriophage systems, primarily studied for its unique role in DNA repair and recombination processes. Originally identified in bacteriophage T4. the UvsE protein functions as a DNA damage-specific endonuclease, playing a critical role in the viral response to ultraviolet (UV) light or chemical-induced DNA lesions. Unlike host cell repair mechanisms, UvsE operates in a replication-coupled manner, selectively incising damaged DNA strands to initiate error-free repair via homologous recombination. This targeted activity has made it a subject of interest in molecular biology and genetic engineering.
Recombinant UvsE is produced through heterologous expression systems, typically in *E. coli*, enabling large-scale purification for research and industrial applications. Its recombinant form retains the enzymatic properties of the native protein, including high specificity for UV-induced cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts. This damage-specific cleavage activity has been harnessed in molecular diagnostics, particularly in DNA damage detection assays and next-generation sequencing protocols to identify lesion sites. Additionally, UvsE has been explored as a tool in synthetic biology for controlled DNA fragmentation or targeted mutagenesis studies.
Recent advancements have highlighted its potential in CRISPR-based technologies, where UvsE's ability to process damaged DNA complements gene-editing workflows. Its thermostability and compatibility with diverse buffer systems further enhance its utility in *in vitro* applications. Researchers also investigate UvsE variants engineered for altered substrate specificity or enhanced catalytic efficiency, expanding its adaptability. Despite its viral origin, the protein’s non-toxic nature and precise enzymatic function position it as a versatile reagent in both basic research and biotechnological innovation, bridging gaps between DNA repair biology and practical genomic engineering tools.
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