| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | uxuA |
| Uniprot No | P24215 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-394aa |
| 氨基酸序列 | MEQTWRWYGP NDPVSLADVR QAGATGVVTA LHHIPNGEVW SVEEILKRKA IIEDAGLVWS VVESVPIHED IKTHTGNYEQ WIANYQQTLR NLAQCGIRTV CYNFMPVLDW TRTDLEYVLP DGSKALRFDQ IEFAAFEMHI LKRPGAEADY TEEEIAQAAE RFATMSDEDK ARLTRNIIAG LPGAEEGYTL DQFRKHLELY KDIDKAKLRE NFAVFLKAII PVAEEVGVRM AVHPDDPPRP ILGLPRIVST IEDMQWMVDT VNSMANGFTM CTGSYGVRAD NDLVDMIKQF GPRIYFTHLR STMREDNPKT FHEAAHLNGD VDMYEVVKAI VEEEHRRKAE GKEDLIPMRP DHGHQMLDDL KKKTNPGYSA IGRLKGLAEV RGVELAIQRA FFSR |
| 预测分子量 | 44,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于uxuA重组蛋白的3篇参考文献及其摘要的模拟示例(注：以下内容为假设性描述，实际文献需根据具体研究检索)：
1. **文献名称**: "Cloning and Heterologous Expression of uxuA Gene Encoding Uronate Isomerase in Escherichia coli"
**作者**: S. Anderson, M. Merrick
**摘要**: 研究报道了从大肠杆菌中克隆uxuA基因，并在重组系统中高效表达，纯化获得活性尿苷酸异构酶，证实其在葡萄糖醛酸代谢途径中的功能。
2. **文献名称**: "Structural and Functional Analysis of Recombinant UxuA Protein from Pseudomonas aeruginosa"
**作者**: H. Jang, R. González
**摘要**: 通过X射线晶体学解析了重组UxuA蛋白的三维结构，揭示了其底物结合位点及催化机制，为设计酶抑制剂提供了结构基础。
3. **文献名称**: "Application of Recombinant UxuA in Biofuel Production via Metabolic Engineering"
**作者**: L. Chen, T. Zhang
**摘要**: 利用重组UxuA优化微生物代谢通路，提升葡萄糖醛酸转化为生物燃料前体的效率，展示了其在可再生能源领域的应用潜力。
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建议通过PubMed、Google Scholar等平台检索真实文献，关键词为“uxuA recombinant protein”或“uronate isomerase expression”。
The uxuA protein, encoded by the uxuA gene in *Escherichia coli* and related Enterobacteriaceae, is a critical enzyme in the galacturonate catabolic pathway. It functions as a mannonate dehydratase, catalyzing the dehydration of D-mannonate to 2-keto-3-deoxy-D-gluconate (KDG), a key step in the utilization of hexuronic acids like glucuronate and galacturonate as carbon sources. This pathway enables bacteria to metabolize plant-derived pectin and algal polysaccharides, supporting their survival in diverse environments.
Recombinant uxuA protein is typically produced via heterologous expression in *E. coli* systems, often fused with affinity tags (e.g., His-tag) to facilitate purification. The protein has a molecular weight of approximately 45-50 kDa and exhibits a conserved structure featuring an α/β barrel fold characteristic of the mandelate racemase enzyme family. Its catalytic mechanism involves metal ion coordination, with magnesium or manganese ions required for enzymatic activity.
Studies of uxuA have provided insights into bacterial carbohydrate metabolism and its role in microbial ecology, particularly in plant-microbe interactions and gut microbiota nutrient cycling. The recombinant enzyme serves as a tool for investigating substrate specificity and reaction kinetics, with potential applications in industrial biotechnology for biomass conversion. Inhibitors targeting uxuA are also explored for antimicrobial strategies against pathogenic Enterobacteriaceae. Recent structural analyses using recombinant uxuA have advanced understanding of enzyme dynamics and metal ion dependency in dehydratase reactions, while its regulatory role in carbon catabolite repression highlights connections to broader metabolic networks.
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