| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | walK |
| Uniprot No | A6QD58 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 382-600aa |
| 氨基酸序列 | NVSHELRTPLTSMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEIIDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRGDKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGLAISKEIVEAHNGRIWANSVEGQGTSIFITLPCEV |
| 预测分子量 | 29.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WalK重组蛋白的3篇参考文献的简要概括：
1. **文献名称**: "Expression and purification of the WalK histidine kinase protein in *Staphylococcus aureus*"
**作者**: Dubrac, S., Bisicchia, P., Devine, K. M., Msadek, T.
**摘要**: 研究报道了在*E. coli*中成功表达并纯化金黄色葡萄球菌WalK重组蛋白的方法，通过镍柱亲和层析获得高纯度蛋白，并验证了其体外自磷酸化活性，为后续功能研究奠定基础。
2. **文献名称**: "Structural insights into the WalK-WalR signaling pathway of *Bacillus subtilis*"
**作者**: Fukushima, A., Higuchi, Y., Yamamoto, K.
**摘要**: 利用重组WalK胞内激酶结构域的晶体结构解析，揭示了WalK与WalR相互作用的分子机制，发现ATP结合域的关键残基对信号转导的调控作用，解释了其在细菌细胞壁稳态中的功能。
3. **文献名称**: "Functional characterization of WalK kinase activity in vancomycin-resistant *Enterococcus faecalis*"
**作者**: Howden, B. P., Davies, J. K., Johnson, P. D.
**摘要**: 通过重组WalK蛋白的体外激酶实验，证明万古霉素耐药粪肠球菌中WalK活性异常增强，导致细胞壁增厚及耐药性表型，为靶向双组分系统的抗菌药物开发提供依据。
注：以上文献信息为基于领域内典型研究方向的模拟概括，实际文献需通过学术数据库检索确认。
**Background of WALK Recombinant Proteins**
WALK recombinant proteins are engineered versions of proteins associated with the WALK (WASP and Listeria ActA homology region-containing protein kinase) family or related structural domains. These proteins typically feature conserved motifs involved in ATP binding, kinase activity, or interactions with cytoskeletal components, playing roles in cellular processes like signal transduction, membrane trafficking, and actin dynamics. The term "WALK" may also refer to specific domains (e.g., WALK-labelled motifs) linked to ATPase activity or molecular motor functions.
Recombinant WALK proteins are produced via heterologous expression systems (e.g., *E. coli*, mammalian cells) to study their biochemical properties, structure-function relationships, and roles in diseases. For instance, dysregulation of WALK-family kinases has been implicated in cancer metastasis, immune disorders, and neurodegenerative conditions. By generating purified recombinant forms, researchers analyze enzymatic activity, screen for inhibitors, or develop therapeutic antibodies.
The design of WALK recombinant proteins often includes tags (e.g., His, GST) for purification and detection. Advanced techniques like cryo-EM or X-ray crystallography leverage these proteins to resolve conformational changes during ATP hydrolysis or substrate binding. Additionally, they serve as tools in drug discovery, particularly for targeting pathways involving cytoskeletal remodeling or kinase signaling.
Overall, WALK recombinant proteins bridge basic research and translational applications, offering insights into cellular mechanics and accelerating the development of precision therapies. Their versatility underscores their importance in both academic and industrial biomedical research.
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