| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | glaB1 |
| Uniprot No | A6KWM0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 15-592aa |
| 氨基酸序列 | CTTLSAQKVYEISAFGLKANSSKNASPVLQKALAKIKAEYKEGEKVILRFPEGRYEFHEKGAAVREYYISNHDQTNPKKVGIALEDMKNLTLDGQGSEFVFHGRMLPVSLLRSENCLLKNFSIDFENPHIAQVKIVENDPQDGIVFEPAPWVDYRIAKDSIFEAYGEGWTMRHSWGIAFDGDTKHLVYNTSDIGCPTKGASEVAPRRIHAPGWKDARLVPGTVVAMRGWGRPTPGIFLSHDVNTTIENVKVHYAEGMGLLAQLCENITLEKFGVCLKGDADPRYFTTQADATHFSGCKGKIVSCNGLYEGMMDDAINVHGTYLKVVKRVDDRTLVGRYMHGQSWGFEWGCPGDEVQFIRSNTMELVGKQNKIISIRPYDKEQTEGAREFLITFQEPVDQVINEQSGFGIENLTWTPEVLFSGNVIRNNRARGSLFSTPRKTIVENNLFDHTSGAAILLCGDCNGWFETGACRHVIIRKNRFVNALTNLFQFTNAVISIYPEIPDLKGQQQYFHGGPEGGIVIEDNEFETFDAPILYAKSVDGLVFRNNTIKLNTEYKPFHPNRNRFWLERVTNVTIAE |
| 预测分子量 | 67.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **glaB1 重组蛋白**的假设参考文献示例(实际文献需根据具体研究补充)：
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1. **文献名称**: *Molecular Cloning and Characterization of glaB1 Gene in Aspergillus oryzae*
**作者**: Tanaka K. et al.
**摘要**: 研究报道了米曲霉中 **glaB1 基因的克隆与功能分析**，发现该基因编码一种热稳定性α-葡萄糖苷酶，其表达受碳源饥饿和高温胁迫的显著诱导，可能在孢子形成中起关键作用。
2. **文献名称**: *Heterologous Expression of glaB1 in Pichia pastoris and Its Enzymatic Properties*
**作者**: Zhang L. et al.
**摘要**: 通过毕赤酵母系统实现 **glaB1 重组蛋白的高效表达与纯化**，酶学性质分析表明其最适反应温度为60°C，pH 5.0.且在淀粉降解工业中具有潜在应用价值。
3. **文献名称**: *Structural Insights into glaB1 Recombinant Protein by X-ray Crystallography*
**作者**: Smith J.R. et al.
**摘要**: 利用X射线晶体学解析了 **glaB1 重组蛋白的三维结构**，揭示了其底物结合口袋的关键氨基酸残基，为理性改造提升催化效率提供了结构基础。
4. **文献名称**: *Application of glaB1 Recombinant Enzyme in Food Industry*
**作者**: Wang Y. et al.
**摘要**: 评估了 **glaB1 重组蛋白在麦芽糖生产中的应用**，证明其较传统酶制剂具有更高的转化率和热稳定性，适用于工业化大规模生产。
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**注**：以上为示例文献，实际文献需通过数据库(如PubMed、SciHub)检索真实发表的研究。如需具体文献，请提供更多研究背景或关键词。
**Background of GlaB1 Recombinant Protein**
GlaB1 recombinant protein is a genetically engineered form of the α-galactosidase B (GlaB1) enzyme, which belongs to the glycoside hydrolase family. This enzyme plays a critical role in catalyzing the hydrolysis of α-linked galactose residues from glycolipids, glycoproteins, and polysaccharides. Naturally occurring in various organisms, including plants, fungi, and bacteria, α-galactosidases are essential for metabolic processes such as breaking down complex carbohydrates. In humans, deficiencies in α-galactosidase A (a related isoform) are linked to Fabry disease, a lysosomal storage disorder. While GlaB1 is structurally and functionally distinct, its recombinant form has garnered interest for biotechnological and therapeutic applications.
The production of GlaB1 recombinant protein typically involves cloning the *glaB1* gene into expression vectors, followed by heterologous expression in systems like *E. coli*, yeast, or mammalian cell cultures. This allows large-scale production of the enzyme with high purity and activity. Recombinant GlaB1 retains the substrate specificity and catalytic efficiency of its native counterpart, making it valuable in industrial processes, such as modifying plant-derived sugars in food technology or enhancing biomass conversion in biofuel production.
In biomedical research, GlaB1 recombinant protein is studied for its potential in enzyme replacement therapy (ERT) for metabolic disorders, though its primary focus remains distinct from Fabry disease-related applications. Additionally, it serves as a tool in glycobiology to analyze glycan structures or synthesize specific oligosaccharides. Challenges in its utilization include optimizing stability, reducing immunogenicity in therapeutic contexts, and improving cost-effective production methods. Ongoing research aims to engineer GlaB1 variants with enhanced properties, broadening its applicability in both industrial and medical fields.
Overall, GlaB1 recombinant protein exemplifies the intersection of enzymology and genetic engineering, offering versatile solutions across diverse scientific and commercial domains.
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