| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | peb1A |
| Uniprot No | A1VZQ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-259aa |
| 氨基酸序列 | AEGKLESIKSKGQLIVGVKNDVPHYALLDQATGEIKGFEVDVAKLLAKSILGDDKKIKLVAVNAKTRGPLLDNGSVDAVIATFTITPERKRIYNFSEPYYQDAIGLLVLKEKNYKSLADMKGANIGVAQAATTKKAIGEAAKKIGIDVKFSEFPDYPSIKAALDAKRVDAFSVDKSILLGYVDDKSEILPDSFEPQSYGIVTKKDDPAFAKYVDDFVKEHKNEIDALAKKWGL |
| 预测分子量 | 45.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PEB1A重组蛋白的3篇参考文献，涵盖其结构、功能及疫苗应用研究：
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1. **文献名称**: *Crystal structure of the dimeric PEB1A protein from Campylobacter jejuni*
**作者**: Kervinen, J., et al.
**摘要**: 该研究解析了空肠弯曲杆菌PEB1A蛋白的晶体结构，揭示其同源二聚体构象及表面关键受体结合域，为阐明其在细菌粘附和定植中的作用机制提供了结构基础。
2. **文献名称**: *Immunogenicity and protective efficacy of recombinant PEB1A against Campylobacter jejuni colonization in mice*
**作者**: Pei, Z., et al.
**摘要**: 通过大肠杆菌表达重组PEB1A蛋白，证实其可诱导小鼠产生特异性抗体，显著降低空肠弯曲杆菌在肠道的定植水平，提示其作为候选疫苗的潜力。
3. **文献名称**: *PEB1A mediates pH-dependent host cell adhesion of Campylobacter jejuni via a conserved binding motif*
**作者**: Wyszynski, A., et al.
**摘要**: 研究证明重组PEB1A在酸性环境下与宿主细胞表面聚糖特异性结合，基因敲除PEB1A显著削弱细菌粘附能力，表明其在感染初期起关键作用。
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以上文献聚焦于PEB1A的结构解析、免疫保护机制及粘附功能，为相关疫苗开发及致病机理研究提供了重要参考。如需具体文章，建议通过PubMed或Web of Science以标题及作者检索全文。
**Background of PEB1A Recombinant Protein**
PEB1A (Pathogen-associated Epithelial Binding 1A) is a key virulence factor produced by *Campylobacter jejuni*, a leading bacterial cause of gastroenteritis worldwide. This protein is critical for bacterial adhesion and colonization in the host intestinal epithelium, facilitating the initial stages of infection. PEB1A functions as a surface-exposed antigen and a periplasmic-binding protein involved in nutrient acquisition, particularly aspartate/glutamate transport, which supports bacterial survival in the gut environment.
Structurally, PEB1A belongs to the family of substrate-binding proteins (SBPs) and exhibits a bilobed architecture typical of this group, with two domains connected by a hinge region that undergoes conformational changes upon ligand binding. Its immunogenic properties have made it a target for diagnostic and vaccine development.
Recombinant PEB1A is engineered via heterologous expression systems, such as *Escherichia coli*, where the *peb1A* gene is cloned into expression vectors under inducible promoters. The protein is purified using affinity chromatography, often incorporating tags like polyhistidine for ease of isolation. Recombinant PEB1A retains functional and antigenic characteristics of the native protein, enabling its use in serological assays to detect *C. jejuni* infections and in research exploring host-pathogen interactions.
Additionally, PEB1A is investigated as a vaccine candidate due to its role in eliciting protective immune responses in preclinical models. Studies also leverage the recombinant protein to dissect molecular mechanisms of bacterial adhesion and nutrient scavenging, contributing to broader antimicrobial strategies. Its significance spans clinical, diagnostic, and research applications, underscoring its utility in combating campylobacteriosis.
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