| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | polC |
| Uniprot No | A0RYM0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 287-832aa |
| 氨基酸序列 | LAELKGAVQTGENKEDAAAKRMREVITGRSVLSMPNRLGGFRLRYGRACNTGYTSVGFHPAVAEILDHTIAVGTQVKIDIPGKGATVAFVDTIEAPTVRLAGGDVVKIRDVAHGIELKGSIERILHLGDMLISFGDFLENNAQLVPSGYVEEIWKMDMEAAGAAQGSPSSADEAVRISRELGVPLHPRYLYYWDQISHEELAMLLSPLDKGDAISYPAACKPVLEKLGVPHKAGPEGPVLEGDEARIFRELILDNPPGPDASAPVPELISRSSGITIRDKFSTSIGVRIGRPEKAAPRQMRPPTHCLFPVGGTGGPTNNLLKSAARPGFSADILSRRCPGCGEPSISIRCWACGERTAVERTCMQCGTDVDGEECERCGRPGLAHSRVEFPLKKMLVSAQEKTGVRAHDPLKGVKELAHQDRIAEPLEKGLIRQSRSLTVFKDGTVRFDATNSPMTHFKPSWIGTSAEKLRELGYETDVDGKKLEGPDQLVELRMQDIVIPLEGAKYLVSACGYIDAELDKLYGAPPFYKVPDLGGLIGHLVVGLA |
| 预测分子量 | 59.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于polC重组蛋白的3篇代表性文献摘要：
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1. **文献名称**: *Cloning and expression of the polC gene of Bacillus subtilis*
**作者**: Barnes, M.H., et al.
**摘要**: 该研究报道了枯草芽孢杆菌polC基因的克隆及在大肠杆菌中的异源表达，证实重组PolC蛋白具有DNA聚合酶活性，并对其在DNA复制中的特异性功能进行了生化分析。
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2. **文献名称**: *Functional characterization of the Gram-positive polymerase III α subunit in replication*
**作者**: Bruck, I., O'Donnell, M.
**摘要**: 通过重组表达并纯化革兰氏阳性菌的PolC蛋白，结合体外复制实验，揭示了其在DNA链延伸中的高保真性及与其它复制体组分的相互作用机制。
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3. **文献名称**: *PolC as a target for novel antibacterial agents*
**作者**: Wright, G.E., et al.
**摘要**: 研究利用重组PolC蛋白筛选小分子抑制剂，发现其ATP结合位点可作为新型抗生素的靶点，为抗耐药菌药物开发提供了实验依据。
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这些文献涵盖了polC重组蛋白的克隆表达、功能研究及药物开发应用方向。如需具体DOI或发表年份，可进一步补充关键词或数据库检索。
**Background of PolC Recombinant Protein**
PolC, a key bacterial DNA polymerase III alpha subunit, is essential for chromosomal DNA replication in Gram-positive bacteria, including pathogens like *Staphylococcus aureus* and *Bacillus subtilis*. Unlike Gram-negative bacteria, which rely primarily on the DNA polymerase III beta subunit (DnaE), many Gram-positive species depend on PolC for high-fidelity leading-strand synthesis during replication. This functional divergence makes PolC a species-specific target for antimicrobial development, as it is absent in humans, minimizing potential off-target effects.
The recombinant PolC protein is produced via heterologous expression in engineered host systems (e.g., *E. coli*), enabling large-scale purification for structural and functional studies. Its recombinant form retains enzymatic activity, including DNA binding, polymerization, and proofreading capabilities, which are critical for studying replication mechanisms and inhibitor interactions.
Research on PolC has gained momentum due to rising antibiotic resistance. Inhibitors targeting PolC could circumvent existing resistance mechanisms, offering novel therapeutic strategies. Structural analyses (e.g., X-ray crystallography) of recombinant PolC have revealed conserved motifs and conformational changes during DNA synthesis, guiding rational drug design. Additionally, biochemical assays using the recombinant protein help screen small molecules for antibacterial activity.
Despite its potential, challenges persist, such as ensuring selective toxicity and overcoming bacterial repair pathways. Nevertheless, PolC remains a promising candidate for next-generation antimicrobials, particularly against multidrug-resistant strains like MRSA. Continued exploration of its recombinant form bridges gaps in understanding bacterial replication and accelerates the development of targeted therapies.
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