| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | US11 |
| Uniprot No | P0A7R9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-129aa |
| 氨基酸序列 | AKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHNGCRPPKKRRV |
| 预测分子量 | 17.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于US11重组蛋白的模拟参考文献示例(注：部分信息为假设性概括，实际文献需通过学术数据库核实)：
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1. **标题**: "Structural Characterization of the Herpes Simplex Virus US11 Protein and Its RNA-Binding Activity"
**作者**: Smith, J.R., et al.
**摘要**: 本研究解析了HSV-1 US11重组蛋白的晶体结构，揭示了其通过C端结构域与宿主RNA结合的能力，为阐明US11在病毒免疫逃逸中的作用机制提供了结构基础。
2. **标题**: "US11 Recombinant Protein Inhibits PKR Activation to Enhance Viral Replication In Vitro"
**作者**: Lee, H., & Williams, C.
**摘要**: 通过在大肠杆菌中表达US11重组蛋白，研究发现其能直接抑制宿主PKR激酶活性，阻断干扰素通路，从而促进病毒在宿主细胞内的复制效率。
3. **标题**: "Expression and Functional Analysis of Human Cytomegalovirus US11 in Eukaryotic Systems"
**作者**: Garcia, M., et al.
**摘要**: 在哺乳动物细胞中表达HCMV US11重组蛋白，证实其通过促进MHC-I类分子降解削弱宿主抗原呈递，为设计靶向US11的抗病毒策略提供了依据。
4. **标题**: "US11 Recombinant Protein as a Potential Vaccine Candidate Against HSV-2 Infection"
**作者**: Zhang, Y., et al.
**摘要**: 评估了US11重组蛋白在小鼠模型中的免疫原性，结果显示其可诱导特异性T细胞应答，提示其在HSV-2亚单位疫苗开发中的潜在应用价值。
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建议通过PubMed或Google Scholar以关键词**"US11 recombinant protein HSV"**或**"US11 protein function"**检索真实文献。
US11 is a virally encoded protein produced by herpes simplex virus (HSV), a pathogen known for causing oral and genital herpes. As part of the viral tegument, US11 plays a critical role in immune evasion, enabling HSV to establish lifelong latency in host neurons. It functions as a multifunctional RNA-binding protein, interfering with host antiviral responses. Notably, US11 inhibits the protein kinase R (PKR)-mediated antiviral pathway by binding to double-stranded RNA (dsRNA) or directly interacting with PKR, thereby suppressing the phosphorylation of eukaryotic initiation factor 2α (eIF2α) and maintaining viral protein synthesis. Additionally, it modulates apoptosis by interacting with cellular proteins like Ras-GAP SH3 domain-binding protein (G3BP1), disrupting stress granule formation and promoting viral replication.
Recombinant US11 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) for structural and functional studies. Its production allows researchers to dissect molecular mechanisms underlying HSV immune evasion, aiding in antiviral drug design. Studies using recombinant US11 have also explored its potential as a therapeutic target or adjuvant due to its immunomodulatory properties. Furthermore, it serves as a tool to investigate host-virus interactions, particularly in understanding how HSV subverts RNA-processing pathways. Research on US11 contributes to broader insights into herpesvirus pathogenesis and the development of strategies to combat persistent viral infections.
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