| 纯度 | >90%SDS-PAGE. |
| 种属 | Staphylococcus |
| 靶点 | entC1 |
| Uniprot No | P01553 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-266aa |
| 氨基酸序列 | ESQPDPTPDELHKASKFTGLMENMKVLYDDHYVSATKVKSVDKFLAHDLIYNISDKKLKNYDKVKTELLNEGLAKKYKDEVVDVYGSNYYVNCYFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLIRVYENKRNTISFEVQTDKKSVTAQELDIKARNFLINKKNLYEFNSSPYETGYIKFIENNGNTFWYDMMPAPGDKFDQSKYLMMYNDNKTVDSKSVKIEVHLTTKNG |
| 预测分子量 | 43.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于entC1重组蛋白的3篇参考文献的示例(注：由于实际文献中可能较少直接提及“entC1”，以下内容基于假设和相关领域研究推测，建议通过学术数据库核实)：
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1. **文献名称**: *Cloning and Heterologous Expression of the entC Gene for Enterobactin Synthesis in Escherichia coli*
**作者**: Smith J, et al.
**摘要**: 本研究克隆了大肠杆菌中的entC基因，并在异源宿主中成功表达重组EntC蛋白。该蛋白被鉴定为异分支酸合酶(isochorismate synthase)，催化enterobactin生物合成的关键步骤。研究验证了重组蛋白的酶活性和在铁载体合成途径中的功能。
2. **文献名称**: *Structural and Functional Analysis of EntC1 in Bacterial Siderophore Production*
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析了重组EntC1蛋白的三维结构，揭示了其底物结合位点和催化机制。功能实验表明，EntC1在病原菌的铁摄取中起重要作用，为抗菌药物靶点研究提供了依据。
3. **文献名称**: *Optimization of Recombinant EntC1 Expression in Bacillus subtilis for Industrial Applications*
**作者**: Zhang R, et al.
**摘要**: 报道了在枯草芽孢杆菌中优化EntC1重组表达的策略，包括启动子选择和发酵条件调控。研究提高了蛋白产量，并证明其在生物合成工程中的潜在应用价值。
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**备注**：若实际文献不足，建议：
1. 确认基因命名准确性(如是否应为entC而非entC1);
2. 扩展检索词(如“enterobactin synthase recombinant”或“siderophore biosynthesis entC”);
3. 查阅综述文章或相关专利引用文献。
EntC1 is a recombinant protein derived from the entC gene, which encodes a key enzyme involved in the biosynthesis of enterobactin, a high-affinity iron-chelating siderophore produced by *Escherichia coli* and other Gram-negative bacteria. Siderophores like enterobactin play a critical role in bacterial iron acquisition, enabling pathogens to scavenge limited iron resources from host environments during infection. The entC gene is part of the *entCEBA* operon responsible for converting chorismic acid into 2.3-dihydroxybenzoate (DHB), a precursor for enterobactin assembly. Specifically, EntC1 functions as an isomerase, catalyzing the conversion of isochorismate to 2.3-dihydro-2.3-dihydroxybenzoate during the DHB synthesis pathway.
Recombinant EntC1 is typically produced by cloning the entC gene into expression vectors (e.g., pET or pGEX systems) and overexpressing it in heterologous hosts like *E. coli*. This allows for high-yield purification and characterization of the enzyme. Studies on EntC1 have focused on elucidating its structure-function relationships, substrate specificity, and regulatory mechanisms, providing insights into bacterial iron metabolism and virulence. Its role in enterobactin production makes it a potential target for antimicrobial strategies, as disrupting iron uptake could attenuate bacterial pathogenicity. Additionally, recombinant EntC1 is used in biochemical assays to screen for inhibitors or to engineer synthetic siderophore pathways in biotechnological applications. Recent structural analyses, including X-ray crystallography, have revealed details about its active site and catalytic residues, aiding rational drug design. Research on EntC1 also contributes to understanding antibiotic resistance mechanisms, as siderophore-mediated iron acquisition is linked to bacterial survival under stress. Overall, EntC1 serves as a model system for studying microbial metallobiology and developing novel therapeutic interventions.
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