| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ppx |
| Uniprot No | P0AFL7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-513aa |
| 氨基酸序列 | PIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQRVHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFLKRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPILVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASGTIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLAILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQARRVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNSDLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEESTPEIAA |
| 预测分子量 | 62.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于重组蛋白研究领域常见方向的示例性参考文献(注：PPX蛋白的命名可能有多种解释，以下内容为假设性概括)：
1. **《Functional Characterization of Recombinant PPX Exopolyphosphatase from Escherichia coli》**
- 作者：Smith A, et al.
- 摘要：本研究克隆并表达了来自大肠杆菌的PPX外切多磷酸酶重组蛋白，分析了其酶学特性及对多磷酸盐底物的降解活性，揭示了其在细菌磷代谢中的关键作用。
2. **《Structural Insights into PPX/GppA Phosphatase Family via Crystallography》**
- 作者：Chen L, et al.
- 摘要：通过X射线晶体学解析了重组PPX蛋白的三维结构，阐明了其催化活性位点及底物结合机制，为设计靶向抑制剂提供了结构基础。
3. **《Heterologous Expression of PPX in Yeast for Industrial Phosphate Removal Applications》**
- 作者：Zhang Y, et al.
- 摘要：优化了PPX重组蛋白在毕赤酵母中的表达条件，证明其在废水处理中高效降解多磷酸盐的应用潜力，为环保技术开发提供新策略。
4. **《PPX Recombinant Protein as a Novel Tool for Polyphosphate Quantification》**
- 作者：Kim T, et al.
- 摘要：开发了一种基于重组PPX酶的比色法检测多磷酸盐，验证其在生物样本中定量分析的灵敏度和特异性，拓展了其在生物检测领域的应用。
**建议**：实际文献需通过学术数据库(如PubMed、Web of Science)以“PPX recombinant protein”“exopolyphosphatase expression”等关键词检索，并结合具体研究背景筛选。
**Background of PPX Recombinant Proteins**
Recombinant proteins, engineered through genetic modification, are produced by inserting target genes into host organisms (e.g., *E. coli*, yeast, or mammalian cells*) to express specific proteins. PPX (exopolyphosphatase), a key enzyme involved in inorganic polyphosphate (polyP) metabolism, catalyzes the hydrolysis of long-chain polyP into shorter chains or inorganic phosphate. Native PPX is found in diverse organisms, including bacteria, archaea, and eukaryotes, playing roles in phosphate homeostasis, stress responses, and biofilm formation. However, isolating PPX from natural sources often yields low quantities and purity, limiting research and industrial applications.
Recombinant PPX technology emerged to address these challenges. By cloning the *ppx* gene into expression vectors, scientists achieve scalable, high-purity production of PPX in controlled systems. This approach allows customization, such as adding affinity tags (e.g., His-tags) for simplified purification. Recombinant PPX is vital for studying polyP-related cellular processes, including bacterial persistence, eukaryotic apoptosis, and biomineralization. It also holds industrial potential, such as in wastewater treatment (polyP removal) or biofuel production (phosphate recycling).
Despite advantages, optimizing PPX expression requires balancing host compatibility, solubility, and enzymatic activity. Structural studies of recombinant PPX have further elucidated its mechanism, guiding enzyme engineering for enhanced stability or substrate specificity. Overall, recombinant PPX exemplifies how synthetic biology bridges fundamental research and practical innovation, offering insights into cellular biochemistry while enabling sustainable biotechnological solutions.
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