| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | hph |
| Uniprot No | P00557 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-341aa |
| 氨基酸序列 | MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE |
| 预测分子量 | 54.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于HPH重组蛋白的模拟参考文献示例(实际文献请通过学术数据库查询)：
---
1. **文献名称**：*Expression and purification of hygromycin phosphotransferase (HPH) recombinant protein in E. coli for antibiotic selection studies*
**作者**：Zhang, L. et al.
**摘要**：研究报道了在大肠杆菌中高效表达HPH重组蛋白的优化方法，通过密码子优化和诱导条件调整，显著提高了蛋白可溶性。该蛋白在体外验证了其磷酸转移酶活性，为抗性筛选系统提供了基础数据。
---
2. **文献名称**：*Development of a mammalian cell line stably expressing HPH-fusion proteins for long-term gene editing applications*
**作者**：Kim, S. & Patel, R.
**摘要**：作者构建了基于HPH与CRISPR-Cas9融合的重组蛋白系统，利用HPH的潮霉素抗性筛选稳定表达细胞株。实验证明该系统在人类HEK293细胞中实现了持续基因编辑能力，且蛋白稳定性提升。
---
3. **文献名称**：*Comparative analysis of HPH and neomycin resistance markers in recombinant protein production using Pichia pastoris*
**作者**：García-Ruiz, A. et al.
**摘要**：研究比较了HPH与新霉素抗性系统在毕赤酵母中的重组蛋白表达效率。结果显示，HPH标记的转化效率更高，且对目标蛋白(如抗体片段)的产量无显著负面影响，推荐用于高密度发酵体系。
---
**备注**：以上为模拟示例，实际文献请通过PubMed、Web of Science等平台检索关键词(如“hygromycin phosphotransferase recombinant”、“HPH selection marker”)。如需具体文献协助，可提供更详细的研究方向。
**Background of HPH Recombinant Protein**
The hygromycin B phosphotransferase (HPH) gene, originally isolated from *Escherichia coli*, encodes an enzyme that confers resistance to the antibiotic hygromycin B. Discovered in the 1980s, HPH became a critical tool in molecular biology and genetic engineering due to its ability to inactivate hygromycin B through phosphorylation, neutralizing its inhibitory effects on protein synthesis. This property made HPH a popular selectable marker for eukaryotic and prokaryotic systems, enabling efficient selection of successfully transfected or transformed cells in research and biotechnological applications.
HPH is widely utilized in constructing recombinant plasmids for gene expression studies, gene knockout models, and transgenic organism development. In mammalian cell cultures, plant transformation, and fungal systems, the HPH gene is co-introduced with target genes, allowing researchers to selectively grow cells that have successfully integrated the foreign DNA using hygromycin B-containing media. Compared to other antibiotic resistance markers, HPH offers advantages in systems where alternatives like neomycin resistance (neo) are less effective, particularly in plants and certain mammalian cells.
Recombinant HPH protein production typically involves expressing the gene in microbial hosts (e.g., *E. coli* or yeast) followed by purification via affinity chromatography. Beyond its role as a selection marker, HPH has been studied for its enzymatic mechanisms and potential applications in antibiotic resistance research. However, its use is carefully regulated in genetically modified organisms (GMOs) to prevent environmental release concerns.
Overall, HPH remains a cornerstone in genetic engineering, balancing practicality with the ongoing need for improved selection systems in synthetic biology and therapeutic development.
×
