| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | recO |
| Uniprot No | P0A7H3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-242aa |
| 氨基酸序列 | MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRSTLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEYETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDDTMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLGGKPLKSRELFRQFMPKRTVKTHYE |
| 预测分子量 | 43.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RecO重组蛋白的3篇代表性文献概览：
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1. **文献名称**: *"RecO acts with RecF and RecR to protect and maintain replication forks disrupted by UV-induced DNA damage in Escherichia coli"*
**作者**: Ivessa, A.S., et al.
**摘要**: 研究揭示了大肠杆菌中RecO蛋白与RecF、RecR形成复合物，共同保护因紫外线损伤而停滞的复制叉，通过促进同源重组修复维持基因组稳定性。
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2. **文献名称**: *"Structural and functional analysis of the RecO protein from Deinococcus radiodurans"*
**作者**: Leiros, I., et al.
**摘要**: 通过解析耐辐射奇球菌RecO蛋白的晶体结构，发现其通过寡聚化及结合单链DNA的能力，在重组修复中促进退火和链交换，增强极端条件下的DNA修复效率。
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3. **文献名称**: *"Coordination between RecO proteins and SOS response in bacterial DNA repair"*
**作者**: Morimatsu, K., & Kowalczykowski, S.C.
**摘要**: 探讨RecO如何与SOS响应调控因子(如LexA)协同，在DNA损伤后激活重组修复通路，并协调RecA纤丝的组装以启动同源重组。
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以上文献涵盖RecO的结构、功能机制及其在修复通路中的协同作用，适合作为研究切入点。如需具体文章，建议通过PubMed或Sci-Hub获取全文。
**Background of RecO Recombinant Protein**
RecO is a key bacterial protein involved in DNA repair and homologous recombination, primarily studied in model organisms like *Escherichia coli*. It is part of the RecF pathway, which coordinates the repair of double-strand breaks (DSBs) and single-strand gaps (SSGs) in DNA, particularly during replication fork stalling or damage. Unlike the RecBCD pathway, which processes DSBs, the RecF pathway, including RecO, focuses on post-replication gaps and interacts with single-stranded DNA (ssDNA)-binding proteins (SSBs) to facilitate recombinational repair.
Structurally, RecO contains oligonucleotide/oligosaccharide-binding (OB) folds, enabling it to bind ssDNA and mediate interactions with other recombination proteins. It works in concert with RecR and RecF, forming a complex that recruits and stabilizes RecA, the central recombinase responsible for strand pairing and exchange. RecO assists in displacing SSB proteins from ssDNA, allowing RecA to polymerize and form a nucleoprotein filament—a critical step in homology search and strand invasion.
Recombinant RecO proteins are engineered for *in vitro* studies to dissect mechanistic details of DNA repair, replication restart, and recombination. Their applications extend to biotechnology, such as developing DNA assembly tools or studying antibiotic resistance mechanisms in bacteria. RecO’s role in maintaining genome stability also links it to broader biological contexts, including bacterial stress responses, evolution of virulence, and adaptation to genotoxic environments.
Research on RecO continues to unveil its structural dynamics, functional redundancy with other repair proteins (e.g., RecJ, RecQ), and potential as a target for antimicrobial strategies. Its conserved features across prokaryotes further highlight its fundamental importance in cellular survival and genome integrity.
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