| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | cmk |
| Uniprot No | Q9TXJ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-348aa |
| 氨基酸序列 | MPLFKRRDGSGPAPNATIREKYDFRDVLGTGAFSKVFLAESKSDAGQMYAVKCIDKKALKGKEESLENEIKVLRKLRHNNIVQLFDTYDEKQFVYLVMELVTGGELFDRIVAKGSYTEQDASNLIRQVLEAVGFMHDNGVVHRDLKPENLLYYNQDEDSKIMISDFGLSKTEDSGVMATACGTPGYVAPEVLQQKPYGKAVDVWSIGVIAYILLCGYPPFYDESDANLFAQIIKGEYEFDAPYWDQISDSAKDFITHLMCCDPEARFTCQDALSHPWISGNTAYTHDIHGTVAVHLKKSLAKRNWKKAYNAAAAIRQLQMLRLSSNSNRLQKQASQQQPEPPTPAFHA |
| 预测分子量 | 39,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CMK重组蛋白的虚构参考文献示例(请注意，这些文献为假设性示例，实际研究中请核实具体文献)：
1. **文献名称**: "Expression and Functional Characterization of Recombinant CMK Protein in Escherichia coli"
**作者**: Zhang L, et al.
**摘要**: 研究报道了在大肠杆菌中高效表达CMK重组蛋白的优化方法，通过亲和层析纯化，并验证了其在体外催化磷酸化反应的活性，为酶学研究提供了工具。
2. **文献名称**: "Structural Insights into CMK Recombinant Protein by X-ray Crystallography"
**作者**: Smith J, et al.
**摘要**: 利用X射线晶体学解析了CMK重组蛋白的三维结构，揭示了其活性位点构象，为设计靶向抑制剂奠定了结构基础。
3. **文献名称**: "CMK Recombinant Protein Enhances Immune Response in Murine Models"
**作者**: Tanaka K, et al.
**摘要**: 在小鼠模型中评估了CMK重组蛋白的免疫调节功能，发现其能显著增强Th1型免疫应答，提示其在疫苗佐剂开发中的潜力。
4. **文献名称**: "Large-scale Production of CMK Recombinant Protein Using Baculovirus System"
**作者**: Wang Y, et al.
**摘要**: 开发了基于杆状病毒-昆虫细胞系统的CMK重组蛋白大规模生产工艺，优化后的方法提高了蛋白产量并降低了成本。
**注意**：CMK可能指代不同蛋白(如磷酸核苷激酶、特定激酶等)，建议结合具体研究领域(如代谢、免疫或结构生物学)进一步检索。实际文献请通过PubMed或Google Scholar以“CMK recombinant protein”及相关关键词查询。
**Background of CMK Recombinant Protein**
CMK recombinant protein refers to a genetically engineered form of the CMK protein, typically designed for research, therapeutic, or diagnostic applications. CMK, often associated with calcium/calmodulin-dependent protein kinase or other context-specific biological targets, plays critical roles in cellular signaling pathways, including calcium homeostasis, cell cycle regulation, and immune responses. The development of recombinant CMK leverages advances in molecular biology, such as codon optimization, vector design, and heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*), to produce high-purity, functional proteins with consistent quality.
The motivation behind recombinant CMK production stems from the limitations of native protein extraction, which often yields low quantities, batch variability, and contamination risks. Recombinant technology overcomes these challenges, enabling scalable synthesis, post-translational modifications (when using eukaryotic systems), and customization (e.g., tagging for purification or tracking). CMK recombinant proteins are widely utilized in structural studies, drug discovery, and mechanistic research to elucidate its role in diseases like cancer, neurodegenerative disorders, or cardiovascular conditions.
Furthermore, CMK recombinant variants are explored for therapeutic potential, including enzyme replacement therapies or as targets for small-molecule inhibitors. The protein's specificity in modulating downstream pathways makes it a valuable tool for developing precision medicine strategies. Ongoing research focuses on optimizing expression systems, enhancing stability, and minimizing immunogenicity for clinical applications. As a cornerstone of modern biotechnology, CMK recombinant protein exemplifies the integration of genetic engineering and biomedical innovation to address unmet scientific and medical needs.
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