| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | cry1Ab |
| Uniprot No | P0A370 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1022-1155aa |
| 氨基酸序列 | HEIENNTDELKFSNCVEEEVYPNNTVTCNDYTATQEEYEGTYTSRNRGYDGAYESNSSVPADYASAYEEKAYTDGRRDNPCESNRGYGDYTPLPAGYVTKELEYFPETDKVWIEIGETEGTFIVDSVELLLMEE |
| 预测分子量 | 17.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Cry1Ab重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control"*
**作者**：Schnepf, E., et al.
**摘要**：本文系统综述了Bt Cry蛋白(包括Cry1Ab)的作用机制，指出其通过与昆虫中肠上皮细胞的特异性受体结合，形成孔洞破坏细胞膜，导致靶标昆虫死亡。重点讨论了Cry1Ab在转基因作物抗虫应用中的潜力。
2. **文献名称**：*"Specific binding of Cry1Ab insecticidal protein from Bacillus thuringiensis to midgut membranes of the rice stem borer (Chilo suppressalis)"*
**作者**：Hua, G., et al.
**摘要**：研究通过体外实验证实，重组Cry1Ab蛋白能与水稻螟虫中肠细胞膜上的氨肽酶N受体特异性结合，解释了其对靶标昆虫的选择毒性，并揭示了抗性昆虫中受体突变导致蛋白结合能力下降的机制。
3. **文献名称**：*"Safety assessment of recombinant Cry1Ab protein in genetically modified crops: degradation in simulated gastric fluid and acute oral toxicity in mice"*
**作者**：Betz, F.S., et al.
**摘要**：通过模拟胃液实验和小鼠急性毒性试验评估重组Cry1Ab的安全性，发现该蛋白在胃液中快速降解且对哺乳动物无显著毒性，支持其在转基因作物中的安全应用。
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**备注**：以上文献为示例，实际引用时需核实具体卷期及发表年份。若需全文，建议通过Web of Science或PubMed以关键词“Cry1Ab recombinant protein”检索最新研究。
The Cry1Ab protein is a delta-endotoxin derived from *Bacillus thuringiensis* (Bt), a soil-dwelling bacterium widely used in agricultural biocontrol. As a member of the Cry toxin family, Cry1Ab exhibits specific insecticidal activity against lepidopteran pests, such as corn borers and cotton bollworms, by targeting their midgut epithelial cells. The protein is produced in crystalline form during bacterial sporulation. Upon ingestion by susceptible insects, Cry1Ab is solubilized in the alkaline midgut environment and proteolytically activated. The activated toxin binds to specific receptors, including cadherin-like proteins and aminopeptidases, forming pores in the cell membrane that disrupt ion balance and cause cell lysis, ultimately leading to insect death.
Recombinant Cry1Ab refers to the protein engineered through genetic modification, where the *cry1Ab* gene is inserted into heterologous expression systems (e.g., *E. coli*, yeast, or plants) for large-scale production. This technology has enabled its widespread use in transgenic crops, such as Bt corn and cotton, to reduce pesticide reliance. Compared to wild-type Bt sprays, recombinant Cry1Ab offers prolonged field stability and tissue-specific expression in plants. However, its ecological impact, potential effects on non-target organisms, and insect resistance evolution remain debated. Studies have focused on optimizing Cry1Ab expression, enhancing toxicity through domain modifications, and developing resistance management strategies. Recent advances include fusion proteins combining Cry1Ab with other Bt toxins to broaden target spectra and delay resistance. Safety assessments confirm its specificity to insects and minimal risk to mammals, underpinning its regulatory approval in many countries. Ongoing research explores Cry1Ab’s interactions with insect immunity and synergism with secondary metabolites to improve sustainable pest control.
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