| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD52 |
| Uniprot No | P43351 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418aa |
| 氨基酸序列 | MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALRQRLGPE YISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNN GKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAVTDG LKRALRSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQDLEPSV EEARYNSCRPNMALGHPQLQQVTSPSRPSHAVIPADQDCSSRSLSSSAVE SEATHQRKLRQKQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPPVTHST PVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAVTPDAGDGVVKPSSRAD PAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTTGN WESHRKSQDMKKRKYDPS |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAD52重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Homologous DNA pairing by RAD52 protein is stimulated by single-strand DNA-binding protein*
**作者**：Sung, P. & Robberson, D.L.
**摘要**：研究发现RAD52在单链DNA结合蛋白(如RPA)存在下，显著增强其介导的同源DNA配对活性，揭示了RAD52在DNA双链断裂修复中与辅助因子协同作用的分子机制。
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2. **文献名称**：*RAD52 facilitates mitotic DNA synthesis following replication stress*
**作者**：Bhowmick, R. et al.
**摘要**：阐明RAD52在复制压力下通过促进断裂诱导复制(BIR)和微同源介导的修复机制，维持基因组稳定性，尤其在BRCA突变细胞中发挥替代性修复通路作用。
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3. **文献名称**：*RAD52 prevents excessive replication fork reversal and promotes restart*
**作者**：Bhat, K.P. & Cortez, D.
**摘要**：揭示RAD52通过阻止复制叉过度逆转(regression)并促进停滞复制叉的重新启动，在S期应对DNA损伤时发挥关键功能，其缺失导致染色体断裂累积。
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如需更多文献或扩展内容，可进一步补充。
RAD52 is a conserved eukaryotic protein central to DNA repair and genome maintenance, primarily through its role in homologous recombination (HR). Discovered in yeast, it facilitates the repair of double-strand breaks (DSBs), critical lesions that can lead to genomic instability if unrepaired. RAD52 functions as a recombination mediator, promoting annealing of complementary DNA strands and stabilizing single-stranded DNA (ssDNA) during HR. Structurally, it forms oligomeric ring complexes that bind ssDNA, enabling homology search and strand pairing—key steps in recombination.
In yeast, RAD52 is essential for HR, compensating for the absence of BRCA2. a critical mediator in higher eukaryotes. However, mammalian RAD52 exhibits functional divergence. While not essential under normal conditions, it becomes crucial in BRCA1/BRCA2-deficient contexts, highlighting a synthetic lethal relationship. This has spurred interest in targeting RAD52 for cancer therapy, particularly in tumors with BRCA mutations or homologous recombination deficiency (HRD).
RAD52 also participates in alternative DNA repair pathways, including single-strand annealing (SSA) and break-induced replication (BIR). Its involvement in SSA, a mutagenic repair mechanism, underscores its dual role in preserving genome stability and potentially contributing to oncogenic rearrangements. Additionally, RAD52 interacts with other repair proteins, such as RAD51. to regulate recombination efficiency and suppress toxic recombination intermediates.
Despite its conserved structure, RAD52's functional importance varies across species, reflecting evolutionary adaptations in DNA repair mechanisms. Ongoing research aims to elucidate its regulatory mechanisms, post-translational modifications, and therapeutic potential. Small-molecule inhibitors of RAD52 are under exploration to exploit synthetic lethality in BRCA-deficient cancers, offering a promising avenue for precision oncology. Understanding RAD52's multifaceted roles continues to inform strategies for combating genomic instability and improving cancer treatments.
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