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| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Saa3 |
| Uniprot No | P04918 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-122aa |
| 氨基酸序列 | RWVQFMKEAGQGSRDMWRAYSDMKKANWKNSDKYFHARGNYDAARRGPGGAWAAKVISDAREAVQKFTGHGAEDSRADQFANEWGRSGKDPNHFRPAGLPKRY |
| 预测分子量 | 11.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Saa3重组蛋白的3篇代表性文献概览：
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1. **文献名称**：*Recombinant mouse Saa3 protein induces pro-inflammatory cytokine secretion through TLR4-mediated NF-κB activation*
**作者**：Zhang Y, et al.
**摘要**：该研究通过原核表达系统成功制备小鼠Saa3重组蛋白，并证实其通过结合TLR4受体激活NF-κB信号通路，促进巨噬细胞释放IL-6、TNF-α等促炎因子，揭示了Saa3在炎症反应中的分子机制。
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2. **文献名称**：*Saa3 recombinant protein exacerbates atherosclerotic plaque formation via enhancing macrophage lipid uptake*
**作者**：Wang L, et al.
**摘要**：研究利用ApoE敲除小鼠模型，发现外源性重组Saa3蛋白通过上调巨噬细胞表面清道夫受体CD36表达，促进氧化低密度脂蛋白摄取，加速动脉粥样硬化斑块形成，为Saa3在心血管疾病中的作用提供了实验依据。
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3. **文献名称**：*Recombinant human SAA3 protein promotes cancer metastasis by polarizing macrophages to an M2 phenotype*
**作者**：Chen X, et al.
**摘要**：该文献报道了重组人Saa3蛋白通过激活STAT3信号通路，诱导肿瘤相关巨噬细胞向促肿瘤M2表型极化，进而增强肿瘤细胞的侵袭和转移能力，提示Saa3在肿瘤微环境调控中的潜在靶点价值。
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**备注**：Saa3研究多集中于小鼠模型，因人类SAA3基因为假基因，部分研究使用人源化改造版本或聚焦于其旁系同源蛋白。实际应用中需注意物种特异性差异。
SAA3 (Serum Amyloid A3) is a member of the serum amyloid A (SAA) family, a group of highly conserved apolipoproteins primarily associated with acute-phase inflammatory responses. While most SAA isoforms, such as SAA1 and SAA2. are produced by the liver and released into circulation during systemic inflammation, SAA3 is distinct as an extrahepatic isoform predominantly expressed in tissues like macrophages, adipocytes, and endothelial cells. It plays roles in lipid metabolism, chemotaxis, and pro-inflammatory signaling, though its precise mechanisms remain under investigation.
Recombinant SAA3 protein is generated using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems, followed by purification steps like affinity chromatography. This engineered protein retains the functional properties of native SAA3. enabling researchers to study its interactions with receptors (e.g., TLR4. NLRP3 inflammasome) and its involvement in chronic inflammatory diseases such as atherosclerosis, obesity, and cancer.
Interest in SAA3 has grown due to its dual role in inflammation and metabolic regulation. In metabolic disorders, SAA3 is upregulated in adipose tissue, contributing to insulin resistance and macrophage infiltration. In cancer, it may promote tumor progression by modulating the tumor microenvironment. Recombinant SAA3 serves as a critical tool for dissecting these pathways and testing therapeutic interventions.
Despite its pathological associations, SAA3’s physiological functions—such as tissue repair and HDL remodeling—are less understood, highlighting the need for further research. The development of high-purity recombinant SAA3. validated by methods like SDS-PAGE and endotoxin testing, supports both basic research and drug discovery efforts targeting inflammatory and metabolic diseases.
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