| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UVSX |
| Uniprot No | P04529 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-391aa |
| 氨基酸序列 | MSDLKSRLIKASTSKLTAELTASKFFNEKDVVRTKIPMMNIALSGEITGG MQSGLLILAGPSKSFKSNFGLTMVSSYMRQYPDAVCLFYDSEFGITPAYL RSMGVDPERVIHTPVQSLEQLRIDMVNQLDAIERGEKVVVFIDSLGNLAS KKETEDALNEKVVSDMTRAKTMKSLFRIVTPYFSTKNIPCIAINHTYETQ EMFSKTVMGGGTGPMYSADTVFIIGKRQIKDGSDLQGYQFVLNVEKSRTV KEKSKFFIDVKFDGGIDPYSGLLDMALELGFVVKPKNGWYAREFLDEETG EMIREEKSWRAKDTNCTTFWGPLFKHQPFRDAIKRAYQLGAIDSNEIVEA EVDELINSKVEKFKSPESKSKSAADLETDLEQLSDMEEFNE |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UVSX重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"In vitro recombination by bacteriophage T4 proteins UvsX and gp32*"
**作者**: Formosa T, Alberts BM
**摘要**: 该研究解析了噬菌体T4的UvsX重组酶与单链DNA结合蛋白gp32协同作用机制，证明其能在体外催化DNA链交换反应，为理解同源重组机制提供模型。
2. **文献名称**: *"UvsX recombinase and mobile crispr spacers impede natural transformation of Vibrio cholerae*"
**作者**: Gunderson FF, Cianciotto NP
**摘要**: 发现霍乱弧菌中UVSX重组酶通过干扰外源DNA整合抑制自然转化，揭示了其在细菌CRISPR适应性免疫系统中的潜在调控作用。
3. **文献名称**: *"Accelerated DNA detection by tandem recombination and polymerase amplification*"
**作者**: Li J, et al.
**摘要**: 开发了一种基于UvsX重组酶的快速核酸检测技术，通过重组酶-聚合酶扩增(RPA)实现常温下病原体DNA快速检测，灵敏度达单分子级别。
注：UVSX相关研究多集中于噬菌体/细菌系统，近年研究侧重其基因编辑应用开发。如需补充可扩展其结构解析类文献(如冷冻电镜研究)。
UVSX recombinant protein is a key component derived from bacteriophage recombination systems, primarily studied for its role in facilitating homologous DNA recombination in vitro. Originally identified in bacteriophage T4. the UVSX gene encodes a protein that functions analogously to bacterial RecA, promoting strand exchange between homologous DNA molecules—a critical step in DNA repair and genetic recombination. This protein has garnered significant attention in molecular biology and genetic engineering due to its ability to enhance the efficiency of DNA assembly and editing workflows.
Structurally, UVSX forms helical filaments on single-stranded DNA (ssDNA), enabling it to search for homology in double-stranded DNA (dsDNA) and catalyze strand invasion. Unlike some eukaryotic recombinases, UVSX operates without requiring ATP hydrolysis, relying instead on ssDNA-binding proteins like UVSY for stabilization. This ATP-independent mechanism simplifies its application in synthetic biology tools, making it particularly useful for in vitro DNA manipulation techniques such as Gibson Assembly and CRISPR-based genome editing enhancements.
Recent advancements have leveraged UVSX in CRISPR-Cas systems to improve homology-directed repair (HDR) efficiency, addressing a major bottleneck in precise genome editing. Its thermostability and compatibility with diverse experimental conditions further broaden its utility in diagnostic assays, synthetic genome construction, and high-throughput DNA cloning. Researchers continue to explore engineered variants of UVSX to optimize activity, specificity, and compatibility with emerging gene-editing technologies, positioning it as a versatile tool for next-generation biomanufacturing and therapeutic development.
×
