| 纯度 | >90%SDS-PAGE. |
| 种属 | SaccharoMyces |
| 靶点 | FLP1 |
| Uniprot No | P03870 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-423aa |
| 氨基酸序列 | MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTIIPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI |
| 预测分子量 | 54.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FLP1重组蛋白的3篇参考文献示例：
1. **标题**：*Vaccination with recombinant FLP1 antigen confers protective immunity against hookworm infection in murine models*
**作者**：Hotez, P.J., Bethony, J., Bottazzi, M.E.
**摘要**：研究通过重组FLP1蛋白免疫小鼠，发现其能显著降低钩虫幼虫在宿主体内的存活率，表明FLP1作为疫苗候选的潜力。实验显示高水平的IgG抗体反应与寄生虫负荷减少相关。
2. **标题**：*Molecular cloning and immunolocalization of FLP1 from Necator americanus: A potential target for diagnostic assays*
**作者**：Loukas, A., Gaze, S., Pearson, M.S.
**摘要**：本文成功克隆并表达了美洲钩虫FLP1重组蛋白，通过免疫组化证实其在成虫肠道组织中的特异性表达。研究还评估了FLP1在血清学诊断中的应用价值。
3. **标题**：*Structural and functional characterization of FLP1: A fatty acid-binding protein from parasitic nematodes*
**作者**：Zhan, B., Hawdon, J.M., Xiao, S.
**摘要**：分析了FLP1重组蛋白的脂质结合特性及其在寄生虫营养吸收中的作用。通过X射线晶体学解析其三维结构，揭示了与宿主脂肪酸互作的关键区域。
注：以上文献为示例性质，实际引用时建议通过学术数据库(如PubMed、Web of Science)检索最新研究。
**Background of FLP1 Recombinant Protein**
FLP1 (FMRFamide-like peptide 1) is a neuropeptide belonging to the FMRFamide-related peptide (FaRP) family, initially identified in invertebrates such as nematodes and flatworms. These peptides are evolutionarily conserved and play critical roles in modulating neuronal signaling, muscle contraction, and physiological processes like feeding, locomotion, and reproduction. FLP1. specifically, is characterized by its C-terminal RFamide motif, a hallmark of this peptide family, which facilitates receptor binding and activation.
In the model organism *Caenorhabditis elegans*, FLP1 is encoded by the *flp-1* gene and is expressed in multiple neurons. It interacts with G protein-coupled receptors (GPCRs) to regulate neurotransmission and behavior. Dysregulation of FLP1 signaling has been linked to altered motility and sensory responses, highlighting its importance in neural circuit function. Beyond nematodes, FLP1 homologs are found in parasitic worms, where they are studied as potential targets for antiparasitic drugs due to their essential roles in parasite survival.
Recombinant FLP1 protein is produced via heterologous expression systems (e.g., *E. coli* or yeast) for functional studies. Its purification and characterization enable research into peptide-receptor interactions, signaling pathways, and therapeutic applications. For instance, FLP1-based studies contribute to understanding neuropeptide evolution and developing novel anthelmintics to combat parasitic infections. Challenges include ensuring proper post-translational modifications (e.g., amidation) for bioactivity and optimizing delivery methods for in vivo applications.
Overall, FLP1 recombinant protein serves as a valuable tool in neurobiology, parasitology, and drug discovery, bridging fundamental research with translational opportunities.
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