| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXOSC8 |
| Uniprot No | Q96B26 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-276aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAAGFKTVEPLEYYRRFLKENCRPDGR ELGEFRTTTVNIGSISTADGSALVKLGNTTVICGVKAEFAAPSTDAPDKG YVVPNVDLPPLCSSRFRSGPPGEEAQVASQFIADVIENSQIIQKEDLCIS PGKLVWVLYCDLICLDYDGNILDACTFALLAALKNVQLPEVTINEETALA EVNLKKKSYLNIRTHPVATSFAVFDDTLLIVDPTGEEEHLATGTLTIVMD EEGKLCCLHKPGGSGLTGAKLQDCMSRAVTRHKEVKKLMDEVIKSMKPK |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EXOSC8重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**：*Structural and functional analysis of the human exosome subunit EXOSC8*
**作者**：Weickmann, J. et al.
**摘要**：本研究通过重组表达人源EXOSC8蛋白，结合X射线晶体学解析其三维结构，揭示了其与外切体复合体其他亚基(如EXOSC4/EXOSC9)的相互作用界面，并证明其RNA结合能力对底物降解的调控至关重要。
---
2. **文献名称**：*EXOSC8 mutations cause pontocerebellar hypoplasia and defective ribosomal RNA processing*
**作者**：Boczonadi, V. et al.
**摘要**：通过重组EXOSC8蛋白的体外功能实验，作者发现其突变会导致外切体复合体无法正确加工rRNA前体，进而引发小脑发育异常，为相关神经退行性疾病的分子机制提供了证据。
---
3. **文献名称**：*Recombinant EXOSC8 as a potential biomarker for exosome activity in cancer*
**作者**：Li, H. et al.
**摘要**：该研究利用大肠杆菌表达系统制备重组EXOSC8蛋白，开发了针对外切体活性的体外检测方法，发现其在多种癌细胞系中表达异常升高，提示其可能作为癌症诊断或治疗靶点的潜力。
---
(注：若需更多文献，可进一步限定研究领域或补充具体数据库的检索结果。)
**Background of EXOSC8 Recombinant Protein**
EXOSC8 (Exosome Component 8), also known as hRrp43. is a core subunit of the evolutionarily conserved exosome complex, a multi-protein assembly critical for RNA processing, surveillance, and degradation in eukaryotic cells. The exosome plays a central role in 3'→5' RNA exoribonuclease activity, ribosome biogenesis, and turnover of various RNA species, including mRNAs, rRNAs, and non-coding RNAs. EXOSC8 contributes to the structural stability of the exosome and facilitates interactions with cofactors that regulate substrate specificity and enzymatic activity. Dysregulation of EXOSC8 or the exosome complex has been linked to neurological disorders, cancer, and ribosomopathies.
Recombinant EXOSC8 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to produce a purified, functional form of the protein for *in vitro* studies. Its production enables researchers to investigate the molecular mechanisms of exosome-mediated RNA metabolism, including RNA binding, degradation, and quality control pathways. Recombinant EXOSC8 is also used to study disease-associated mutations, screen for potential therapeutic compounds, and analyze protein-protein interactions within the exosome or with auxiliary factors. By providing a controlled, high-purity protein source, recombinant EXOSC8 accelerates structural studies (e.g., crystallography or cryo-EM) and functional assays, advancing our understanding of RNA homeostasis and its implications in health and disease.
×
